Cell numbers and subsets generated from wt and TNF/LTα/LTβ−/− BM culture
. | CD11c+, I-Ab low-high (× 105cells/well) . | CD11c−, I-Ab− (× 105 cells/well) . |
---|---|---|
wt | 3.65 ± 0.67 | 0.40 ± 0.07 |
TNF/LTα/LTβ−/− | 2.36 ± 0.29* | 1.59 ± 0.20* |
. | CD11c+, I-Ab low-high (× 105cells/well) . | CD11c−, I-Ab− (× 105 cells/well) . |
---|---|---|
wt | 3.65 ± 0.67 | 0.40 ± 0.07 |
TNF/LTα/LTβ−/− | 2.36 ± 0.29* | 1.59 ± 0.20* |
BM cells (1 × 106 cells/mL) from wt or TNF/LTα/LTβ−/− mice were cultured with GM-CSF and IL-4. Cells were collected on day 9 and stained with FITC-conjugated anti–I-Ab mAb and PE-conjugated anti-CD11c mAb. The total cell numbers were counted, and the number of each subset was calculated by each percentage. Numbers are the results from 4 experiments ± SD.
P < .01 compared with wt BM culture.