Table 1.

Cell numbers and subsets generated from wt and TNF/LTα/LTβ−/− BM culture

CD11c+, I-Ab low-high
(× 105cells/well)
CD11c, I-Ab−
(× 105 cells/well)
wt 3.65 ± 0.67 0.40 ± 0.07 
TNF/LTα/LTβ−/− 2.36 ± 0.29* 1.59 ± 0.20* 
CD11c+, I-Ab low-high
(× 105cells/well)
CD11c, I-Ab−
(× 105 cells/well)
wt 3.65 ± 0.67 0.40 ± 0.07 
TNF/LTα/LTβ−/− 2.36 ± 0.29* 1.59 ± 0.20* 

BM cells (1 × 106 cells/mL) from wt or TNF/LTα/LTβ−/− mice were cultured with GM-CSF and IL-4. Cells were collected on day 9 and stained with FITC-conjugated anti–I-Ab mAb and PE-conjugated anti-CD11c mAb. The total cell numbers were counted, and the number of each subset was calculated by each percentage. Numbers are the results from 4 experiments ± SD.

*

P < .01 compared with wt BM culture.

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