Addition of exogenous hemin restores erythroid differentiation in DMSO-treated 745A/Bcl-XL AS cells
| Inducer . | Benzidine-positive cells, % . | |||
|---|---|---|---|---|
| Day 4 . | Day 5 . | |||
| 745A/Neo . | 745A/Bcl-XLAS . | 745A/Neo . | 745A/Bcl-XL AS . | |
| None | 0 | 0 | 0 | 0 |
| Hemin | 1 | 1 | 1 | 1 |
| DMSO | 80 | 1 | 85 | 1 |
| DMSO + hemin | 97 | 6 | 98 | 48 |
| Inducer . | Benzidine-positive cells, % . | |||
|---|---|---|---|---|
| Day 4 . | Day 5 . | |||
| 745A/Neo . | 745A/Bcl-XLAS . | 745A/Neo . | 745A/Bcl-XL AS . | |
| None | 0 | 0 | 0 | 0 |
| Hemin | 1 | 1 | 1 | 1 |
| DMSO | 80 | 1 | 85 | 1 |
| DMSO + hemin | 97 | 6 | 98 | 48 |
745A/Neo and 745A/Bcl-XLAS cells were treated with hemin (100 μM), DMSO (2%), or DMSO plus hemin. Cultures were scored for erythroid differentiation by benzidine staining after 4 or 5 days of treatment.