SDF-1–induced migration ability of CD34+ and IBMI-CD34− SRCs
| Transplanted cells . | Transplantation route . | Incidence of engraftment . | Human CD45+ cells (%)* . |
|---|---|---|---|
| Lin−CD34high | |||
| Migrating | IBMI | 5/5 | 0.7-15.2 (5.5) |
| Migrating | TVI | 5/5 | 0.1-2.4 (0.3) |
| Nonmigrating | IBMI | 5/5 | 0.8-12.5 (2.5) |
| Nonmigrating | TVI | 1/5 | 0.1 |
| Lin−CD34− | |||
| Migrating | IBMI | 0/3 | 0 |
| Nonmigrating | IBMI | 3/3 | 0.5-3.5 (3.1) |
| Transplanted cells . | Transplantation route . | Incidence of engraftment . | Human CD45+ cells (%)* . |
|---|---|---|---|
| Lin−CD34high | |||
| Migrating | IBMI | 5/5 | 0.7-15.2 (5.5) |
| Migrating | TVI | 5/5 | 0.1-2.4 (0.3) |
| Nonmigrating | IBMI | 5/5 | 0.8-12.5 (2.5) |
| Nonmigrating | TVI | 1/5 | 0.1 |
| Lin−CD34− | |||
| Migrating | IBMI | 0/3 | 0 |
| Nonmigrating | IBMI | 3/3 | 0.5-3.5 (3.1) |
Immunomagnetically separated cells were allowed to migrate toward a gradient of SDF-1 as described in “Materials and methods.” After the transwell migration, both migrating and nonmigrating cells were recovered. These cells were sorted into Lin−CD34high and Lin−CD34− cells. Cells (5 × 103 Lin−CD34high and 5 × 104 Lin−CD34−) were then transplanted into NOD/SCID mice by IBMI or TVI, as indicated. After 12 weeks, repopulation of human CD45+ cells in murine BMs was analyzed.
Median values are presented in parentheses.