Probe and primer combinations used for the quantitative real-time PCR
| Gene . | Sequence . |
|---|---|
| dCK | |
| Forward | 5′-TGC AGG GAA GTC AAC ATT-3′ |
| Reverse | 5′-TCC CAC CAT TTT TCT GAG-3′ |
| Probe | 5′-(FAM)-TAA ACA ATT GTG TGA AGA TTG GGA AG-(TAMRA)-3′ |
| CDA | |
| Forward | 5′-GGA GGC CAA GAA GTC AG-3′ |
| Reverse | 5′-GAC GGC CTT CTG GAT AG-3′ |
| Probe | 5′-(FAM)-CAA CAT AGA AAA TGC CTG CTA CCC-(TAMRA)-3′ |
| dCMPD | |
| Forward | 5′-AAT GGG TGC AGT GAT GAC-3′ |
| Reverse | 5′-CTT AGC GCA TTC ATT ACA AG-3′ |
| Probe | 5′-(FAM)-ATC ATG AAC AAA AAT TCG ACC GAT-(TAMRA)-3′ |
| PN-I | |
| Forward | 5′-AAT CGG CGA TGT ACT AGA G-3′ |
| Reverse | 5′-CAT CTG CCA TTC TTA AGT CTC-3′ |
| Probe | 5′-(FAM)-ATG AAA CTG GGG TGC TCA AAG GA-(TAMRA)-3′ |
| hENT1 | |
| Forward | 5′-TGT TTC CAG CCG TGA CT-3′ |
| Reverse | 5′-CAG GCC ACA TGA ATA CAG-3′ |
| Probe | 5′-(FAM)-CAG CAC CTG GGA ACG TTA CTT-(TAMRA)-3′ |
| GAPDH | |
| Forward | 5′-GTC GGA GTC AAC GGA TT-3′ |
| Reverse | 5′-AAG CTT CCC GTT CTC AG-3′ |
| Probe | 5′-(FAM)-TCA ACT ACA TGG TTT ACA TGT TCC AA-(TAMRA)-3′ |
| Gene . | Sequence . |
|---|---|
| dCK | |
| Forward | 5′-TGC AGG GAA GTC AAC ATT-3′ |
| Reverse | 5′-TCC CAC CAT TTT TCT GAG-3′ |
| Probe | 5′-(FAM)-TAA ACA ATT GTG TGA AGA TTG GGA AG-(TAMRA)-3′ |
| CDA | |
| Forward | 5′-GGA GGC CAA GAA GTC AG-3′ |
| Reverse | 5′-GAC GGC CTT CTG GAT AG-3′ |
| Probe | 5′-(FAM)-CAA CAT AGA AAA TGC CTG CTA CCC-(TAMRA)-3′ |
| dCMPD | |
| Forward | 5′-AAT GGG TGC AGT GAT GAC-3′ |
| Reverse | 5′-CTT AGC GCA TTC ATT ACA AG-3′ |
| Probe | 5′-(FAM)-ATC ATG AAC AAA AAT TCG ACC GAT-(TAMRA)-3′ |
| PN-I | |
| Forward | 5′-AAT CGG CGA TGT ACT AGA G-3′ |
| Reverse | 5′-CAT CTG CCA TTC TTA AGT CTC-3′ |
| Probe | 5′-(FAM)-ATG AAA CTG GGG TGC TCA AAG GA-(TAMRA)-3′ |
| hENT1 | |
| Forward | 5′-TGT TTC CAG CCG TGA CT-3′ |
| Reverse | 5′-CAG GCC ACA TGA ATA CAG-3′ |
| Probe | 5′-(FAM)-CAG CAC CTG GGA ACG TTA CTT-(TAMRA)-3′ |
| GAPDH | |
| Forward | 5′-GTC GGA GTC AAC GGA TT-3′ |
| Reverse | 5′-AAG CTT CCC GTT CTC AG-3′ |
| Probe | 5′-(FAM)-TCA ACT ACA TGG TTT ACA TGT TCC AA-(TAMRA)-3′ |