PCR primers for gene expression analyses
| Genes . | Primer sequences . | Annealing temperature, °C . | Reference/ source . |
|---|---|---|---|
| VJ558-JH4 | S: CAGGTCCAACTGCAGCAG | 67 | Li et al23 |
| A: CTCTCAGCCGGCTCCCTCAGGG | |||
| PU.1 | S: CGGATGACTTGGTTACTTACG | 62 | Lin and |
| A: GTAGGAAACCTGGTGACTGAG | Grosschedl24 | ||
| E12 | S: CGCACTGACCACGAGCTTCAC | 63 | Li et al25 |
| A: GGTCGTCCTCGTCCTCGTCTG | |||
| E47 | S: CGCACTGACCACGAGCTTCAC | 62 | Li et al25 |
| A: TCCAGGGACAGCACCTCATCTG | |||
| EBF | S: GCCAACAGCGAAAAGACC | 62 | J.G.M.'s lab |
| A: GCTTGGAGTTATTGTGGAC | |||
| Pax5 | S: CTACAGGCTCCGTGACGCAG | 61 | J.G.M.'s lab |
| A: GTCTCGGCCTGTGACAATAGG | |||
| N-myc | S: CGGTGGCTGCTCCTGCTCGTG | 64 | Nagy et al26 |
| A: TGTCCTGCCCCCTACCCAGAT | |||
| MB1 | S: GCCAGGGGGTCTAGAAGC | 66 | Li et al23 |
| A: TCACTTGGCACCCAGTACAA |
| Genes . | Primer sequences . | Annealing temperature, °C . | Reference/ source . |
|---|---|---|---|
| VJ558-JH4 | S: CAGGTCCAACTGCAGCAG | 67 | Li et al23 |
| A: CTCTCAGCCGGCTCCCTCAGGG | |||
| PU.1 | S: CGGATGACTTGGTTACTTACG | 62 | Lin and |
| A: GTAGGAAACCTGGTGACTGAG | Grosschedl24 | ||
| E12 | S: CGCACTGACCACGAGCTTCAC | 63 | Li et al25 |
| A: GGTCGTCCTCGTCCTCGTCTG | |||
| E47 | S: CGCACTGACCACGAGCTTCAC | 62 | Li et al25 |
| A: TCCAGGGACAGCACCTCATCTG | |||
| EBF | S: GCCAACAGCGAAAAGACC | 62 | J.G.M.'s lab |
| A: GCTTGGAGTTATTGTGGAC | |||
| Pax5 | S: CTACAGGCTCCGTGACGCAG | 61 | J.G.M.'s lab |
| A: GTCTCGGCCTGTGACAATAGG | |||
| N-myc | S: CGGTGGCTGCTCCTGCTCGTG | 64 | Nagy et al26 |
| A: TGTCCTGCCCCCTACCCAGAT | |||
| MB1 | S: GCCAGGGGGTCTAGAAGC | 66 | Li et al23 |
| A: TCACTTGGCACCCAGTACAA |
S indicates sense; A, antisense.