Effect of neutrophil serine proteases on CFU-GM growth
| . | No. colonies/5 × 104 BMMCc ± SD (inhibition %) . | ||||
|---|---|---|---|---|---|
| Control . | Elastase (%) . | Proteinase 3 (%) . | Cathepsin G (%) . | Azurocidin (%) . | |
| 10 μg/mL | |||||
| Donor 1 | 140 ± 22 | 82 ± 50 (41) | 136 ± 20 (9) | 122 ± 38 (13) | 134 ± 50 (5) |
| Donor 2 | 130 ± 2 | 90 ± 26 (31) | 98 ± 14 (24) | 126 ± 10 (4) | 136 ± 2 (−4) |
| Donor 3 | 107 ± 7 | 80 ± 6 (26) | 108 ± 5 (−1) | ND | ND |
| Donor 4 | 228 ± 6 | 161 ± 4 (29) | 228 ± 3 (0) | ND | ND |
| Inhibition, mean % | 32 | 8 | 9 | 1 | |
| 1 μg/mL | |||||
| Donor 1 | — | 116 ± 50 (17) | 158 ± 36 (−12) | 134 ± 34 (4) | 136 ± 12 (3) |
| Donor 2 | — | 98 ± 12 (25) | 84 ± 24 (36) | 126 ± 8 (4) | 128 ± 8 (2) |
| Donor 3 | — | 74 ± 9 (31) | 109 ± 5 (−2) | ND | ND |
| Donor 4 | — | 148 ± 17 (35) | 225 ± 8 (1) | ND | ND |
| Inhibition, mean % | 27 | 6 | 4 | 3 | |
| 0.1 μg/mL | |||||
| Donor 1 | — | 158 ± 22 (−13) | 148 ± 24 (−5) | 146 ± 16 (−4) | 150 ± 26 (−7) |
| Donor 2 | — | 106 ± 6 (19) | 88 ± 26 (31) | 120 ± 14 (8) | 136 ± 6 (−4) |
| Donor 3 | — | 72 ± 3 (33) | 107 ± 10 (0) | ND | ND |
| Donor 4 | — | 183 ± 7 (20) | 225 ± 10 (1) | ND | ND |
| Inhibition, mean % | 15 | 7 | 2 | −6 | |
| 0.01 μg/mL | |||||
| Donor 1 | — | 128 ± 92 (9) | 144 ± 48 (−3) | 136 ± 54 (2) | 122 ± 50 (13) |
| Donor 2 | — | 94 ± 2 (28) | 94 ± 18 (28) | 128 ± 12 (2) | 126 ± 14 (4) |
| Donor 3 | — | 103 ± 11 (4) | 100 ± 6 (7) | ND | ND |
| Donor 4 | — | 228 ± 3 (0) | 230 ± 2 (−1) | ND | ND |
| Inhibition, mean % | 10 | 8 | 2 | 9 | |
| . | No. colonies/5 × 104 BMMCc ± SD (inhibition %) . | ||||
|---|---|---|---|---|---|
| Control . | Elastase (%) . | Proteinase 3 (%) . | Cathepsin G (%) . | Azurocidin (%) . | |
| 10 μg/mL | |||||
| Donor 1 | 140 ± 22 | 82 ± 50 (41) | 136 ± 20 (9) | 122 ± 38 (13) | 134 ± 50 (5) |
| Donor 2 | 130 ± 2 | 90 ± 26 (31) | 98 ± 14 (24) | 126 ± 10 (4) | 136 ± 2 (−4) |
| Donor 3 | 107 ± 7 | 80 ± 6 (26) | 108 ± 5 (−1) | ND | ND |
| Donor 4 | 228 ± 6 | 161 ± 4 (29) | 228 ± 3 (0) | ND | ND |
| Inhibition, mean % | 32 | 8 | 9 | 1 | |
| 1 μg/mL | |||||
| Donor 1 | — | 116 ± 50 (17) | 158 ± 36 (−12) | 134 ± 34 (4) | 136 ± 12 (3) |
| Donor 2 | — | 98 ± 12 (25) | 84 ± 24 (36) | 126 ± 8 (4) | 128 ± 8 (2) |
| Donor 3 | — | 74 ± 9 (31) | 109 ± 5 (−2) | ND | ND |
| Donor 4 | — | 148 ± 17 (35) | 225 ± 8 (1) | ND | ND |
| Inhibition, mean % | 27 | 6 | 4 | 3 | |
| 0.1 μg/mL | |||||
| Donor 1 | — | 158 ± 22 (−13) | 148 ± 24 (−5) | 146 ± 16 (−4) | 150 ± 26 (−7) |
| Donor 2 | — | 106 ± 6 (19) | 88 ± 26 (31) | 120 ± 14 (8) | 136 ± 6 (−4) |
| Donor 3 | — | 72 ± 3 (33) | 107 ± 10 (0) | ND | ND |
| Donor 4 | — | 183 ± 7 (20) | 225 ± 10 (1) | ND | ND |
| Inhibition, mean % | 15 | 7 | 2 | −6 | |
| 0.01 μg/mL | |||||
| Donor 1 | — | 128 ± 92 (9) | 144 ± 48 (−3) | 136 ± 54 (2) | 122 ± 50 (13) |
| Donor 2 | — | 94 ± 2 (28) | 94 ± 18 (28) | 128 ± 12 (2) | 126 ± 14 (4) |
| Donor 3 | — | 103 ± 11 (4) | 100 ± 6 (7) | ND | ND |
| Donor 4 | — | 228 ± 3 (0) | 230 ± 2 (−1) | ND | ND |
| Inhibition, mean % | 10 | 8 | 2 | 9 | |
ND indicates not determined; —, not applicable.