Table 4. FGFR3 expression and detection... | American Society of Hematology

Table 4.

FGFR3 expression and detection of der(14)

Patient	Clinical isotype	BMPC, %	t(4;14) type	FGFR3 expression	Exon 1 der(14) detection (isotype)	Exon 3 der(14) detection (isotype)
1	IgA	32^4-150	MB4-1	Yes	Yes (IgG)	ND
2	IgA	55^4-150	MB4-1	Yes	No	No
3	IgG	70^4-150	MB4-1	Yes	No	ND
4	IgG	15^4-150	MB4-1	Yes	No	ND
5	IgG	55^4-150	MB4-1	Yes	Yes (IgG)	ND
6	IgG	10^4-150	MB4-1	Yes	Yes (IgG)	ND
7	IgG	30^4-150	MB4-1	Yes	Yes (IgG)	ND
8	IgA	40^4-150	MB4-1	Yes	No	ND
9	IgA	14^4-150	MB4-1	Yes	No	ND
10	IgA	33^4-150	MB4-1	Yes	No	ND
11	IgG	40^4-150	MB4-1	Yes	No	ND
12	IgG	69^4-150	MB4-1	Yes	Yes (IgG)	No
13	IgG	35^4-150	MB4-1	Yes	Yes (IgG)	ND
14	Light, κ	15^4-150	MB4-1	Yes	No	ND
15	IgA	45^4-150	MB4-1	Yes	No	ND
16	IgG	12^4-150	MB4-1	Yes	No	ND
17	IgG	30^4-150	MB4-1	Yes	Yes (IgM)	No
18	IgG	37	MB4-1	Yes	No	No
19	IgG	20^4-150	MB4-1	No	No	No
20	IgG	10^4-150	MB4-1	No	No	ND
21	IgG	40^4-150	MB4-1	No	No	No
22	?	10^4-150	MB4-2	Yes	Yes (IgG)	Yes (IgG)
23	IgG	10^4-150	MB4-2	Yes	No	No
24	IgA	50^4-150	MB4-2	Yes	Yes (IgA)	Yes (IgA)
25	IgG	77^4-150	MB4-2	No	No	No
26	Light, κ	68	MB4-2	No	No	No
27	IgA	24	MB4-3	Yes	Yes (IgA)	Yes (IgA)
28	IgG	18	MB4-3	Yes	Yes (IgG)	Yes (IgG)
29	IgG	80^4-150	MB4-3	No	No	No
30	IgA	50^4-150	MB4-3	No	No	No
31	IgG	20^4-150	MB4-3	No	No	No
32	IgM/IgG	10^4-150	MB4-1	ND	Yes (IgG)	ND

Patient	Clinical isotype	BMPC, %	t(4;14) type	FGFR3 expression	Exon 1 der(14) detection (isotype)	Exon 3 der(14) detection (isotype)
1	IgA	32^4-150	MB4-1	Yes	Yes (IgG)	ND
2	IgA	55^4-150	MB4-1	Yes	No	No
3	IgG	70^4-150	MB4-1	Yes	No	ND
4	IgG	15^4-150	MB4-1	Yes	No	ND
5	IgG	55^4-150	MB4-1	Yes	Yes (IgG)	ND
6	IgG	10^4-150	MB4-1	Yes	Yes (IgG)	ND
7	IgG	30^4-150	MB4-1	Yes	Yes (IgG)	ND
8	IgA	40^4-150	MB4-1	Yes	No	ND
9	IgA	14^4-150	MB4-1	Yes	No	ND
10	IgA	33^4-150	MB4-1	Yes	No	ND
11	IgG	40^4-150	MB4-1	Yes	No	ND
12	IgG	69^4-150	MB4-1	Yes	Yes (IgG)	No
13	IgG	35^4-150	MB4-1	Yes	Yes (IgG)	ND
14	Light, κ	15^4-150	MB4-1	Yes	No	ND
15	IgA	45^4-150	MB4-1	Yes	No	ND
16	IgG	12^4-150	MB4-1	Yes	No	ND
17	IgG	30^4-150	MB4-1	Yes	Yes (IgM)	No
18	IgG	37	MB4-1	Yes	No	No
19	IgG	20^4-150	MB4-1	No	No	No
20	IgG	10^4-150	MB4-1	No	No	ND
21	IgG	40^4-150	MB4-1	No	No	No
22	?	10^4-150	MB4-2	Yes	Yes (IgG)	Yes (IgG)
23	IgG	10^4-150	MB4-2	Yes	No	No
24	IgA	50^4-150	MB4-2	Yes	Yes (IgA)	Yes (IgA)
25	IgG	77^4-150	MB4-2	No	No	No
26	Light, κ	68	MB4-2	No	No	No
27	IgA	24	MB4-3	Yes	Yes (IgA)	Yes (IgA)
28	IgG	18	MB4-3	Yes	Yes (IgG)	Yes (IgG)
29	IgG	80^4-150	MB4-3	No	No	No
30	IgA	50^4-150	MB4-3	No	No	No
31	IgG	20^4-150	MB4-3	No	No	No
32	IgM/IgG	10^4-150	MB4-1	ND	Yes (IgG)	ND

Each number represents one t(4;14)⁺ patient, and patient 32 is the one MGUS patient detected by the 2-stage assay. The clinical isotype was determined by serum electrophoresis. Plasma cell percentage for the initial BM sample tested was determined by a pathologist. The breakpoint type was determined based on the RT-PCR product size of the IgH-MMSET hybrid assay. Expression of FGFR3 was determined by RT-PCR with 5′ and 3′ FGFR3-C primers. The presence of der(14) products was verified by RT-PCR with 5′ MMSET exon 1 or 5′ MMSET exon 3 primers in combination with either CμB, CδB, CγB, or CαB. The 5′ MMSET exon 3 reaction is only completely reported for MB4-2 and MB4-3 patients. The reaction will only produce a product in these patients because exon 3 remains on der(4) in the MB4-1 patients.

ND indicates not done; and ?, unknown clinical isotype.

F4-150

Diagnostic samples.

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