FGFR3 expression and detection of der(14)
Patient . | Clinical isotype . | BMPC, % . | t(4;14) type . | FGFR3 expression . | Exon 1 der(14) detection (isotype) . | Exon 3 der(14) detection (isotype) . |
---|---|---|---|---|---|---|
1 | IgA | 324-150 | MB4-1 | Yes | Yes (IgG) | ND |
2 | IgA | 554-150 | MB4-1 | Yes | No | No |
3 | IgG | 704-150 | MB4-1 | Yes | No | ND |
4 | IgG | 154-150 | MB4-1 | Yes | No | ND |
5 | IgG | 554-150 | MB4-1 | Yes | Yes (IgG) | ND |
6 | IgG | 104-150 | MB4-1 | Yes | Yes (IgG) | ND |
7 | IgG | 304-150 | MB4-1 | Yes | Yes (IgG) | ND |
8 | IgA | 404-150 | MB4-1 | Yes | No | ND |
9 | IgA | 144-150 | MB4-1 | Yes | No | ND |
10 | IgA | 334-150 | MB4-1 | Yes | No | ND |
11 | IgG | 404-150 | MB4-1 | Yes | No | ND |
12 | IgG | 694-150 | MB4-1 | Yes | Yes (IgG) | No |
13 | IgG | 354-150 | MB4-1 | Yes | Yes (IgG) | ND |
14 | Light, κ | 154-150 | MB4-1 | Yes | No | ND |
15 | IgA | 454-150 | MB4-1 | Yes | No | ND |
16 | IgG | 124-150 | MB4-1 | Yes | No | ND |
17 | IgG | 304-150 | MB4-1 | Yes | Yes (IgM) | No |
18 | IgG | 37 | MB4-1 | Yes | No | No |
19 | IgG | 204-150 | MB4-1 | No | No | No |
20 | IgG | 104-150 | MB4-1 | No | No | ND |
21 | IgG | 404-150 | MB4-1 | No | No | No |
22 | ? | 104-150 | MB4-2 | Yes | Yes (IgG) | Yes (IgG) |
23 | IgG | 104-150 | MB4-2 | Yes | No | No |
24 | IgA | 504-150 | MB4-2 | Yes | Yes (IgA) | Yes (IgA) |
25 | IgG | 774-150 | MB4-2 | No | No | No |
26 | Light, κ | 68 | MB4-2 | No | No | No |
27 | IgA | 24 | MB4-3 | Yes | Yes (IgA) | Yes (IgA) |
28 | IgG | 18 | MB4-3 | Yes | Yes (IgG) | Yes (IgG) |
29 | IgG | 804-150 | MB4-3 | No | No | No |
30 | IgA | 504-150 | MB4-3 | No | No | No |
31 | IgG | 204-150 | MB4-3 | No | No | No |
32 | IgM/IgG | 104-150 | MB4-1 | ND | Yes (IgG) | ND |
Patient . | Clinical isotype . | BMPC, % . | t(4;14) type . | FGFR3 expression . | Exon 1 der(14) detection (isotype) . | Exon 3 der(14) detection (isotype) . |
---|---|---|---|---|---|---|
1 | IgA | 324-150 | MB4-1 | Yes | Yes (IgG) | ND |
2 | IgA | 554-150 | MB4-1 | Yes | No | No |
3 | IgG | 704-150 | MB4-1 | Yes | No | ND |
4 | IgG | 154-150 | MB4-1 | Yes | No | ND |
5 | IgG | 554-150 | MB4-1 | Yes | Yes (IgG) | ND |
6 | IgG | 104-150 | MB4-1 | Yes | Yes (IgG) | ND |
7 | IgG | 304-150 | MB4-1 | Yes | Yes (IgG) | ND |
8 | IgA | 404-150 | MB4-1 | Yes | No | ND |
9 | IgA | 144-150 | MB4-1 | Yes | No | ND |
10 | IgA | 334-150 | MB4-1 | Yes | No | ND |
11 | IgG | 404-150 | MB4-1 | Yes | No | ND |
12 | IgG | 694-150 | MB4-1 | Yes | Yes (IgG) | No |
13 | IgG | 354-150 | MB4-1 | Yes | Yes (IgG) | ND |
14 | Light, κ | 154-150 | MB4-1 | Yes | No | ND |
15 | IgA | 454-150 | MB4-1 | Yes | No | ND |
16 | IgG | 124-150 | MB4-1 | Yes | No | ND |
17 | IgG | 304-150 | MB4-1 | Yes | Yes (IgM) | No |
18 | IgG | 37 | MB4-1 | Yes | No | No |
19 | IgG | 204-150 | MB4-1 | No | No | No |
20 | IgG | 104-150 | MB4-1 | No | No | ND |
21 | IgG | 404-150 | MB4-1 | No | No | No |
22 | ? | 104-150 | MB4-2 | Yes | Yes (IgG) | Yes (IgG) |
23 | IgG | 104-150 | MB4-2 | Yes | No | No |
24 | IgA | 504-150 | MB4-2 | Yes | Yes (IgA) | Yes (IgA) |
25 | IgG | 774-150 | MB4-2 | No | No | No |
26 | Light, κ | 68 | MB4-2 | No | No | No |
27 | IgA | 24 | MB4-3 | Yes | Yes (IgA) | Yes (IgA) |
28 | IgG | 18 | MB4-3 | Yes | Yes (IgG) | Yes (IgG) |
29 | IgG | 804-150 | MB4-3 | No | No | No |
30 | IgA | 504-150 | MB4-3 | No | No | No |
31 | IgG | 204-150 | MB4-3 | No | No | No |
32 | IgM/IgG | 104-150 | MB4-1 | ND | Yes (IgG) | ND |
Each number represents one t(4;14)+ patient, and patient 32 is the one MGUS patient detected by the 2-stage assay. The clinical isotype was determined by serum electrophoresis. Plasma cell percentage for the initial BM sample tested was determined by a pathologist. The breakpoint type was determined based on the RT-PCR product size of the IgH-MMSET hybrid assay. Expression of FGFR3 was determined by RT-PCR with 5′ and 3′ FGFR3-C primers. The presence of der(14) products was verified by RT-PCR with 5′ MMSET exon 1 or 5′ MMSET exon 3 primers in combination with either CμB, CδB, CγB, or CαB. The 5′ MMSET exon 3 reaction is only completely reported for MB4-2 and MB4-3 patients. The reaction will only produce a product in these patients because exon 3 remains on der(4) in the MB4-1 patients.
ND indicates not done; and ?, unknown clinical isotype.
Diagnostic samples.