PCR primers
| Primer . | Sequence, 5′ to 3′ . |
|---|---|
| 5′ β2-M | CCAGCAGAGAATGGAAAGTC |
| 3′ β2-M | GATGCTGCTTACATGTCTCG |
| JH | ACCACGGTCACCGTCTCCTCA |
| Iμ1 | AGCCCTTGTTAATGGACTTG |
| Iμ2 | CTTTGCAAGGCTCGCAGTGAC |
| ms6r | CCTCAATTTCCCTGAAATTGGTT |
| ms5r | AAGAACTGTACGTGATACTG |
| 5′ FGFR3-C | CGGCAGACGTACACGCTG |
| 3′ FGFR3-C | GTGGTGTGTTGGAGCTCATG |
| 5′ MMSET exon 1 | CCGAGGATGCGACGCACCGCAG |
| 5′ MMSET exon 3 | TGTCGAAGCAGCTCTTGTGT |
| CμB | GACGGAATTCTCACAGGAGAC |
| CδB | TGGGTGTCTGCACCCTGATAT |
| CγB | GGGGAAGACCGATGGGCCCT |
| CαB | GAGGCTCAGCGGGAAGACCTT |
| Primer . | Sequence, 5′ to 3′ . |
|---|---|
| 5′ β2-M | CCAGCAGAGAATGGAAAGTC |
| 3′ β2-M | GATGCTGCTTACATGTCTCG |
| JH | ACCACGGTCACCGTCTCCTCA |
| Iμ1 | AGCCCTTGTTAATGGACTTG |
| Iμ2 | CTTTGCAAGGCTCGCAGTGAC |
| ms6r | CCTCAATTTCCCTGAAATTGGTT |
| ms5r | AAGAACTGTACGTGATACTG |
| 5′ FGFR3-C | CGGCAGACGTACACGCTG |
| 3′ FGFR3-C | GTGGTGTGTTGGAGCTCATG |
| 5′ MMSET exon 1 | CCGAGGATGCGACGCACCGCAG |
| 5′ MMSET exon 3 | TGTCGAAGCAGCTCTTGTGT |
| CμB | GACGGAATTCTCACAGGAGAC |
| CδB | TGGGTGTCTGCACCCTGATAT |
| CγB | GGGGAAGACCGATGGGCCCT |
| CαB | GAGGCTCAGCGGGAAGACCTT |
The sequence of primers used to detect β2-microglobin, IgH-MMSET hybrids (der[4]), FGFR3, and MMSET-IgH hybrids (der[14]) are listed in 5′ to 3′ orientation.