Table 2. Observed pre-mRNA intermediates... | American Society of Hematology

Table 2.

Observed pre-mRNA intermediates and deduced preferential order of splicing

Introns	Primer pair	Introns spliced	Size, bp	Order
1 vs 2	A	None	1309	2 before 1
	B	Intron 2	360
2 vs 3	C	None	905	(2 before 3)^†
	D	Intron 2	446	or
		None	1463	3 before 2^†
		Intron 3	331
3 vs 4	E	None	1530	3 before 4
	F	Intron 3	398
		None	1181
1 vs 3	G	None	2052	3 before 1
	H	Intron 2	1593
		Introns 1 + 2^*	524^*
		Introns 2 + 3	402
2 vs 4	I	Introns 2 + 3	524	2 before 4
	J	Intron 3	1245
1 vs 4	K	Intron 3	2130	4 before 1
	L	Introns 1 + 2 + 3	603
		Introns 2 + 3 + 4	731

Introns	Primer pair	Introns spliced	Size, bp	Order
1 vs 2	A	None	1309	2 before 1
	B	Intron 2	360
2 vs 3	C	None	905	(2 before 3)^†
	D	Intron 2	446	or
		None	1463	3 before 2^†
		Intron 3	331
3 vs 4	E	None	1530	3 before 4
	F	Intron 3	398
		None	1181
1 vs 3	G	None	2052	3 before 1
	H	Intron 2	1593
		Introns 1 + 2^*	524^*
		Introns 2 + 3	402
2 vs 4	I	Introns 2 + 3	524	2 before 4
	J	Intron 3	1245
1 vs 4	K	Intron 3	2130	4 before 1
	L	Introns 1 + 2 + 3	603
		Introns 2 + 3 + 4	731

Transcripts that may result from minor pathways of splicing involving intron 1 (see “Discussion”).

The preferential splice order between intron 3 and intron 2 was determined by analyzing nuclear RNA intermediates (Figure 5B).