TNF-α/zVAD-fmk-induced cell death of CGD PMNs
| Treatment . | % Annexin-V+ PMNs . | |
|---|---|---|
| CGD, n = 3 . | Control, n = 3 . | |
| No addition* | 50.2 ± 5.1 | 46.4 ± 3.2 |
| TNF-α | 53.0 ± 4.5 | 59.0 ± 11.0 |
| zVAD-fmk | 35.0 ± 4.3† | 31.2 ± 10.5† |
| TNF-α/zVAD-fmk* | 63.7 ± 12.7‡ | 65.8 ± 9.5‡ |
| NAC + TNF-α/zVAD-fmk | 35.2 ± 1.81-153 | 35.3 ± 3.21-153 |
| Treatment . | % Annexin-V+ PMNs . | |
|---|---|---|
| CGD, n = 3 . | Control, n = 3 . | |
| No addition* | 50.2 ± 5.1 | 46.4 ± 3.2 |
| TNF-α | 53.0 ± 4.5 | 59.0 ± 11.0 |
| zVAD-fmk | 35.0 ± 4.3† | 31.2 ± 10.5† |
| TNF-α/zVAD-fmk* | 63.7 ± 12.7‡ | 65.8 ± 9.5‡ |
| NAC + TNF-α/zVAD-fmk | 35.2 ± 1.81-153 | 35.3 ± 3.21-153 |
PMNs isolated from 3 CGD patients and healthy day controls were incubated for 6 hours (“delayed” culturing; see “Material and methods”) without additions, with 20 ng/mL TNF-α, with 150 μM zVAD-fmk, with the combination of these agents alone, or with addition of 5 mM NAC. Thereafter, PMNs were stained with annexin-V/PI and analyzed by FACScan. Values represent means ± SD of 3 to 5 separate experiments performed in duplicate.
n = 5.
P < .05 versus No addition.
P < .05 versus zVAD-fmk.
P < .05 versus TNF-α/zVAD-fmk, in the respective columns for CGD or control cells.