Influence of fluoxetine on phosphatidylserine exposure and DNA strand breaks in L3055 BL cells
| Treatment† . | Annexin V binding* . | % TUNEL positive‡ . | |
|---|---|---|---|
| % positive . | MFI . | ||
| Control | 52.3 ± 1.8 | 3.9 ± 0.2 | 17.8 ± 3.7 |
| Anti-IgM | 94.5 ± 1.0 | 71.1 ± 12.1 | 51.7 ± 14.5 |
| Fluoxetine | 81.1 ± 3.9 | 26.1 ± 8.4 | 35.7 ± 7.6 |
| Treatment† . | Annexin V binding* . | % TUNEL positive‡ . | |
|---|---|---|---|
| % positive . | MFI . | ||
| Control | 52.3 ± 1.8 | 3.9 ± 0.2 | 17.8 ± 3.7 |
| Anti-IgM | 94.5 ± 1.0 | 71.1 ± 12.1 | 51.7 ± 14.5 |
| Fluoxetine | 81.1 ± 3.9 | 26.1 ± 8.4 | 35.7 ± 7.6 |
Samples analyzed by FACS to generate percent positive cells within the Annexin V+/PI− “apoptotic” gate together with mean fluorescent intensity (MFI) of stain. Results are means ± SEMs of 3 separate experiments.
L3055 cells were cultured at 106/mL for 17 hours in control medium, with anti-IgM (μ chain) at 10 μg/mL or fluoxetine at 20 μM.
Samples analyzed by FACS to generate percent nonsubdiploid cells (as assessed by PI stain) positive by TUNEL. Results are means ± SEs of 3 separate experiments.