Distribution of human hematopoietic cells (CD34+CD38−) in iBM-injected NOD/SCID mice
| No. cells . | Percentage of human CD45+ cells . | |||
|---|---|---|---|---|
| Injected tibia . | Noninjected tibia . | Spleen . | PB . | |
| 10 000 | 86.13 | 57.91 | 64.68 | 27.86 |
| 1 000 | 33.62 | 5.28 | 3.80 | 0.85 |
| 500 | 47.94 | 1.28 | 2.44 | ND |
| 250 | 5.00 | 0.10 | 0.10 | 0.04 |
| 100 | 5.58 | 0.12 | 0.35 | 0.02 |
| No. cells . | Percentage of human CD45+ cells . | |||
|---|---|---|---|---|
| Injected tibia . | Noninjected tibia . | Spleen . | PB . | |
| 10 000 | 86.13 | 57.91 | 64.68 | 27.86 |
| 1 000 | 33.62 | 5.28 | 3.80 | 0.85 |
| 500 | 47.94 | 1.28 | 2.44 | ND |
| 250 | 5.00 | 0.10 | 0.10 | 0.04 |
| 100 | 5.58 | 0.12 | 0.35 | 0.02 |
BM cells, spleen cells, and PB of NOD/SCID mice 8 weeks after transplantation of CB Lin−/lowCD34+CD38− cells by iBM were stained with antihuman CD45 mAbs and analyzed. BM cells were collected separately from tibiae of injected and noninjected side.
ND indicates not determined.