Table 2. Oligonucleotide primers used... | American Society of Hematology

Table 2.

Oligonucleotide primers used for PCR amplification

Genotyping	Sense primer, 5′-3′	Antisense primer, 5′-3′	Annealing temperature, °C	PCR product size, bp
−506 I/D of PAR-I	CTG TCG ACG TCT CCA CAT CCC AGG A	CGA AGC TGT CAG TGA CTC ACA CTG G	58	103/90
IVSn−14 A/T of PAR-1	TTG TCG CTT TTG CCT TGT TG	GGG AGC ACA GAC ACA AAC AG	62	406
−1426 C/T of PAR-1	AAC TGG GTA CTT CCG CAA TTT	TGC TAA GGG CCC CCA GGG GCG TCG CGG CTG GGG GTG GCC	56	430
807 C/T of α₂ integrin	GGG GAC CTC ACA AAC AGA TT	TAA CTT TCC CAG CTG CCT TC	56	271
1565 C/T of GP IIIa	TTC TGA TTG CTG GAC TTC TCT T	TCT CTC CCC ATG GCA AAG AGT	60	266

Genotyping	Sense primer, 5′-3′	Antisense primer, 5′-3′	Annealing temperature, °C	PCR product size, bp
−506 I/D of PAR-I	CTG TCG ACG TCT CCA CAT CCC AGG A	CGA AGC TGT CAG TGA CTC ACA CTG G	58	103/90
IVSn−14 A/T of PAR-1	TTG TCG CTT TTG CCT TGT TG	GGG AGC ACA GAC ACA AAC AG	62	406
−1426 C/T of PAR-1	AAC TGG GTA CTT CCG CAA TTT	TGC TAA GGG CCC CCA GGG GCG TCG CGG CTG GGG GTG GCC	56	430
807 C/T of α₂ integrin	GGG GAC CTC ACA AAC AGA TT	TAA CTT TCC CAG CTG CCT TC	56	271
1565 C/T of GP IIIa	TTC TGA TTG CTG GAC TTC TCT T	TCT CTC CCC ATG GCA AAG AGT	60	266