Table 1. Effect of fixation and... | American Society of Hematology

Table 1.

Effect of fixation and permeabilization on GPA positivity of vesicles in plasma obtained by pheresis (n = 8)

Vesicles	Anti–GPA-PE positive (% ± SD)
Before fixation/permeabilization	65.4 ± 11.7^*
After fixation/permeabilization
In the absence of anti–Hb-FITC	31.7 ± 5.9^†
In the presence of anti–Hb-FITC	29.8 ± 4.6^†

Vesicles	Anti–GPA-PE positive (% ± SD)
Before fixation/permeabilization	65.4 ± 11.7^*
After fixation/permeabilization
In the absence of anti–Hb-FITC	31.7 ± 5.9^†
In the presence of anti–Hb-FITC	29.8 ± 4.6^†

Anti–GPA-PE negative vesicles were nearly all anti-CD41 positive.

Significantly different (P < .0002) from control vesicles (Student paired t test).