Results of immunohistochemical stainings of normal tonsil and lymph node sections of patients with cHL
| B-lineage proteins . | cHL cases . | Cases without protein expression/cases analyzed . | Protein expression in normal lymphoid tissue . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | |||
| RP105 | − | NE | − | − | + | − | − | − | − | NE | − | ND | ND | ND | 8/9 | Strongly in mantle zone cells, weakly in GC cells, some interfollicular cells |
| Lck | NE | − | − | + | − | − | − | − | − | − | − | ND | ND | ND | 9/10 | High in interfollicular cells (probably T cells), GC cells, and mantle zone cells |
| Syk | − | − | − | − | − | − | − | − | − | − | − | ND | ND | ND | 11/11 | GC cells, mantle zone cells, and many interfollicular cells |
| BCMA | NE | − | + | NE | − | − | − | − | − | − | − | + | + | − | 9/12 | GC cells, some mantle zone cells, and single interfollicular cells |
| BLNK | ND | − | NE | NE | − | − | − | − | NE | − | NE | NE | − | − | 8/8 | GC cells and mantle zone cells |
| SWAP-70 | + | − | − | ND | ND | ND | ND | − | − | − | − | ND | ND | ND | 6/7 | Strong in GC cells |
| CD52 | − | − | NE | − | NE | − | − | NE | − | − | − | − | − | − | 11/11 | Many GC cells, mantle zone cells, many interfollicular cells |
| Blk | NE | NE | NE | NE | NE | NE | − | NE | − | NE | NE | NE | − | − | 4/4 | Strong in GC cells, mantle zone, several interfollicular cells |
| SIT | + | − | − | NE | NE | − | − | − | − | − | − | − | − | − | 11/12 | Strong in GC cells, weaker in mantle zone cells and interfollicular cells |
| EBV status | + | + | + | + | + | NE | − | − | − | − | + | ND | ND | ND | NA | NA |
| B-lineage proteins . | cHL cases . | Cases without protein expression/cases analyzed . | Protein expression in normal lymphoid tissue . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | |||
| RP105 | − | NE | − | − | + | − | − | − | − | NE | − | ND | ND | ND | 8/9 | Strongly in mantle zone cells, weakly in GC cells, some interfollicular cells |
| Lck | NE | − | − | + | − | − | − | − | − | − | − | ND | ND | ND | 9/10 | High in interfollicular cells (probably T cells), GC cells, and mantle zone cells |
| Syk | − | − | − | − | − | − | − | − | − | − | − | ND | ND | ND | 11/11 | GC cells, mantle zone cells, and many interfollicular cells |
| BCMA | NE | − | + | NE | − | − | − | − | − | − | − | + | + | − | 9/12 | GC cells, some mantle zone cells, and single interfollicular cells |
| BLNK | ND | − | NE | NE | − | − | − | − | NE | − | NE | NE | − | − | 8/8 | GC cells and mantle zone cells |
| SWAP-70 | + | − | − | ND | ND | ND | ND | − | − | − | − | ND | ND | ND | 6/7 | Strong in GC cells |
| CD52 | − | − | NE | − | NE | − | − | NE | − | − | − | − | − | − | 11/11 | Many GC cells, mantle zone cells, many interfollicular cells |
| Blk | NE | NE | NE | NE | NE | NE | − | NE | − | NE | NE | NE | − | − | 4/4 | Strong in GC cells, mantle zone, several interfollicular cells |
| SIT | + | − | − | NE | NE | − | − | − | − | − | − | − | − | − | 11/12 | Strong in GC cells, weaker in mantle zone cells and interfollicular cells |
| EBV status | + | + | + | + | + | NE | − | − | − | − | + | ND | ND | ND | NA | NA |
Protein expression in normal tissue is given in the right column. No protein expression of Syk, BLNK, or CD52 was detected in HRS cells in any of the cases investigated. Lck, RP105, SIT, and SWAP-70 expression was found in one case each, with only some HRS cells being positive in these cases. For BCMA we found a more diverse pattern as 3 cases of 12 show protein expression in some HRS cells. The EBV status was analyzed by EBER in situ hybridization.
NE indicates not evaluable; ND, not done; NA, not applicable; −, negative; and +, positive.