Down-regulation of B-lineage genes in the L1236 cell line, identified by SAGE, subdivided in B-cell–, lymphoid-, and hematopoietic-specifically expressed genes
| . | Number of tags expressed . | Gene function . | |
|---|---|---|---|
| Centroblasts . | L1236 . | ||
| B-cell–specific genes | |||
| Igβ (CD79b) | 45 | 1 | BCR-associated protein |
| Igα (CD79a) | 21 | 0 | BCR-associated protein |
| OBF | 19 | 0 | Transcription factor |
| CD22 | 16 | 0 | BCR coreceptor |
| SWAP-70 | 9 | 0 | Subunit of the SWAP protein complex |
| CD20 | 8 | 0 | BCR coreceptor |
| RP105 (CD180) | 8 | 0 | Toll-like receptor, LPS receptor |
| CD19 | 8 | 1 | BCR coreceptor |
| Igκ | 7 | 0 | BCR light chain |
| A-myb | 7 | 0 | Transcription factor |
| BLNK | 4 | 0 | B-cell linker protein |
| Lymphoid-specific genes | |||
| LPAP (CD45-AP) | 41 | 0 | CD45-associated protein |
| Alemtuzumab (CD52) | 36 | 0 | GPI-anchored antigen |
| Jaw-1 | 33 | 2 | Integral membrane protein of the endoplasmic reticulum |
| Lck | 6 | 0 | Protein tyrosine kinase |
| Blk | 5 | 0 | Protein tyrosine kinase |
| Spi-B (PU.B) | 5 | 0 | Transcription factor |
| SIT | 4 | 0 | SHP2-interacting protein |
| Hematopoietic-specific genes | |||
| CD53 | 25 | 0 | Tetraspanin |
| CD37 | 19 | 1 | Tetraspanin |
| hICSBP-1 | 17 | 0 | Suggested as a repressor of ICS-containing genes |
| CXCR4 | 15 | 1 | Chemokine receptor |
| c-Src | 15 | 1 | Protein tyrosine kinase |
| HPK-1 | 10 | 1 | STE20-related serine/threonine kinase |
| CD45 | 19 | 2 | Common leucocyte antigen, phosphatase |
| PLC-γ2 | 12 | 1 | Signal transmitter (eg, of receptor tyrosine kinases) |
| CD72 | 8 | 1 | C-type lectin |
| Spi-1 (PU.1) | 6 | 0 | Transcription factor |
| HS1 (HCLS1) | 6 | 0 | Hematopoietic cell–specific Lyn substrate |
| Lyl-1 | 5 | 0 | Transcription factor |
| 47-kDa protein | 5 | 0 | NCF-1 (human 47-kDa autosomal chronic granulomatous disease protein) |
| LFA-1 | 5 | 0 | Leukocyte integrin |
| GPI80 | 3 | 0 | GPI-anchored protein; role in leukocyte trafficking suggested |
| WASP | 3 | 0 | Protein involved in the organization of the actin cytoskeleton |
| Lyn | 3 | 0 | Protein tyrosine kinase |
| . | Number of tags expressed . | Gene function . | |
|---|---|---|---|
| Centroblasts . | L1236 . | ||
| B-cell–specific genes | |||
| Igβ (CD79b) | 45 | 1 | BCR-associated protein |
| Igα (CD79a) | 21 | 0 | BCR-associated protein |
| OBF | 19 | 0 | Transcription factor |
| CD22 | 16 | 0 | BCR coreceptor |
| SWAP-70 | 9 | 0 | Subunit of the SWAP protein complex |
| CD20 | 8 | 0 | BCR coreceptor |
| RP105 (CD180) | 8 | 0 | Toll-like receptor, LPS receptor |
| CD19 | 8 | 1 | BCR coreceptor |
| Igκ | 7 | 0 | BCR light chain |
| A-myb | 7 | 0 | Transcription factor |
| BLNK | 4 | 0 | B-cell linker protein |
| Lymphoid-specific genes | |||
| LPAP (CD45-AP) | 41 | 0 | CD45-associated protein |
| Alemtuzumab (CD52) | 36 | 0 | GPI-anchored antigen |
| Jaw-1 | 33 | 2 | Integral membrane protein of the endoplasmic reticulum |
| Lck | 6 | 0 | Protein tyrosine kinase |
| Blk | 5 | 0 | Protein tyrosine kinase |
| Spi-B (PU.B) | 5 | 0 | Transcription factor |
| SIT | 4 | 0 | SHP2-interacting protein |
| Hematopoietic-specific genes | |||
| CD53 | 25 | 0 | Tetraspanin |
| CD37 | 19 | 1 | Tetraspanin |
| hICSBP-1 | 17 | 0 | Suggested as a repressor of ICS-containing genes |
| CXCR4 | 15 | 1 | Chemokine receptor |
| c-Src | 15 | 1 | Protein tyrosine kinase |
| HPK-1 | 10 | 1 | STE20-related serine/threonine kinase |
| CD45 | 19 | 2 | Common leucocyte antigen, phosphatase |
| PLC-γ2 | 12 | 1 | Signal transmitter (eg, of receptor tyrosine kinases) |
| CD72 | 8 | 1 | C-type lectin |
| Spi-1 (PU.1) | 6 | 0 | Transcription factor |
| HS1 (HCLS1) | 6 | 0 | Hematopoietic cell–specific Lyn substrate |
| Lyl-1 | 5 | 0 | Transcription factor |
| 47-kDa protein | 5 | 0 | NCF-1 (human 47-kDa autosomal chronic granulomatous disease protein) |
| LFA-1 | 5 | 0 | Leukocyte integrin |
| GPI80 | 3 | 0 | GPI-anchored protein; role in leukocyte trafficking suggested |
| WASP | 3 | 0 | Protein involved in the organization of the actin cytoskeleton |
| Lyn | 3 | 0 | Protein tyrosine kinase |
Numbers of tags are indicated for both expression profiles (normalized to 30 000 tags). Igκ is listed as an example for several BCR genes (the L1236 cell line harbors an Igκ rearrangement.16 Some genes such as c-Src, Syk, and PLC-γ are mentioned in the hematopoietic class of genes, despite more ubiquitous expression, because of their well-known functional importance in B-cell signaling. B-lineage genes identified as down-regulated by a difference lower than 3:0 were not listed in the table although identified in the Affymetrix analysis (Syk, MD-1, LPTP, TTF; all show a tag ratio of 2:0). The complete list of differentially expressed genes is available as supplemental data on theBlood website; see the Supplemental Tables link at the top of the online article.