GlcNAcT activities of the enzymes encoded from the mutant IGnTA and IGnTCcDNAs
| . | GlcNAc transferred, pmol . |
|---|---|
| Vector | 26.7 ± 8.5 |
| IGnTA | 143.0 ± 5.4 |
| IGnTA*1049A | 23.2 ± 4.9 |
| IGnTA*1154A | 24.3 ± 3.9 |
| Vector | 7.2 ± 2.3 |
| IGnTC | 210.9 ± 63.5 |
| IGnTC*505A | 24.5 ± 5.1 |
| IGnTC*683A | 15.6 ± 3.0 |
| IGnTC*1049A | 12.6 ± 2.1 |
| IGnTC*1154A | 4.7 ± 6.0 |
| . | GlcNAc transferred, pmol . |
|---|---|
| Vector | 26.7 ± 8.5 |
| IGnTA | 143.0 ± 5.4 |
| IGnTA*1049A | 23.2 ± 4.9 |
| IGnTA*1154A | 24.3 ± 3.9 |
| Vector | 7.2 ± 2.3 |
| IGnTC | 210.9 ± 63.5 |
| IGnTC*505A | 24.5 ± 5.1 |
| IGnTC*683A | 15.6 ± 3.0 |
| IGnTC*1049A | 12.6 ± 2.1 |
| IGnTC*1154A | 4.7 ± 6.0 |
Results are the averages and standard deviations of 4 tests. Endogenous transfer of GlcNAc in the absence of acceptor substrate was corrected for each test.