Classes of selectable marker genes and corresponding drugs
Principle . | Utility . | Example for gene . | Drug/agent category . | Agent . | Reference no. . |
---|---|---|---|---|---|
Surface tag | In vitro | dLNGFR | Monoclonal antibody | NA | 120,121,123, 144-149 |
Drug resistance | In vitro and in vivo | MGMT | Cytotoxic agent | Temozolomide, BCNU | 137, 150-152 |
DHFR | Cytotoxic agent | MTX | 137 | ||
MDR1 | Cytotoxic agent | Paclitaxel, etoposide | 45,46,134,135,139-142 | ||
Growth promoting | In vitro and in vivo | FK-mpl | Chemical dimerizer | AP1903 | 153,154 |
SAG | Inducer of protein function | Estrogens | 155 | ||
HOXB4 | Controlled expression required | NA | 51,125-131 |
Principle . | Utility . | Example for gene . | Drug/agent category . | Agent . | Reference no. . |
---|---|---|---|---|---|
Surface tag | In vitro | dLNGFR | Monoclonal antibody | NA | 120,121,123, 144-149 |
Drug resistance | In vitro and in vivo | MGMT | Cytotoxic agent | Temozolomide, BCNU | 137, 150-152 |
DHFR | Cytotoxic agent | MTX | 137 | ||
MDR1 | Cytotoxic agent | Paclitaxel, etoposide | 45,46,134,135,139-142 | ||
Growth promoting | In vitro and in vivo | FK-mpl | Chemical dimerizer | AP1903 | 153,154 |
SAG | Inducer of protein function | Estrogens | 155 | ||
HOXB4 | Controlled expression required | NA | 51,125-131 |
This table does not provide a comprehensive overview of the genes developed as selectable markers. Its purpose is rather to show the different principles with a few selected examples. NA indicates currently not available as good manufacturing practice product.