Categories of side effects in the genetic manipulation of hematopoietic stem cells
| Level . | Category . | Example . |
|---|---|---|
| 1 | Culture | Loss of homing potential |
| 2 | Vector | Fusiogenic properties of viral envelope proteins |
| 3 | Genotoxicity | Insertional mutagenesis |
| 4 | Phenotoxicity | Interference of transgene product with cellular signaling networks |
| 5 | Selection toxicity | Side effects of regimens used to selectively engraft or expand manipulated cells |
| 6 | Immune response | Elimination of modified transgenic cells by antibodies or cytotoxic T cells directed against transgene-encoded antigens |
| 7 | Interactions of 1-6 | Cooperation of 3, 4, and 5 |
| Level . | Category . | Example . |
|---|---|---|
| 1 | Culture | Loss of homing potential |
| 2 | Vector | Fusiogenic properties of viral envelope proteins |
| 3 | Genotoxicity | Insertional mutagenesis |
| 4 | Phenotoxicity | Interference of transgene product with cellular signaling networks |
| 5 | Selection toxicity | Side effects of regimens used to selectively engraft or expand manipulated cells |
| 6 | Immune response | Elimination of modified transgenic cells by antibodies or cytotoxic T cells directed against transgene-encoded antigens |
| 7 | Interactions of 1-6 | Cooperation of 3, 4, and 5 |