Cytogenetic and FISH findings in 11 NLPHL cases with the 3q27/BCL6 rearrangement
| Case (LN sample) . | Status . | Cytogenetics/3q27 abnormalities . | Interphase FISH patterns found with LSI BCL6 (no. of nuclei) . | Interpretations of FISH results . | Confirmation of BCL6-R . | |
|---|---|---|---|---|---|---|
| Ploidy* . | Status of 3q27/BCL6 . | |||||
| 1 | D | t(3;22)(q27;q11) | 1F1G1O (10) | 2n | 1xchr3, t(3;22)(q27;q11) | IHC/FISH |
| (BCL6/BCL6) | ||||||
| 2(a) | D | der(3)t(3;?;1)(q27;?;q21) | ND | ND | ||
| 2(b) | R | der(3)t(3;?;1)(q27;?;q21) | 2F4G2O (6) | 4n† | 2xchr.3, 2xder(3)t(3;7;3;1)(3pter_q27::7p12→7pter:: 3q27::7p12::1q21→1qter), 2xder(7)t(3;7)(q27;p12)† | ND |
| 3 | D | NK | 1F1G1O (3) | 2n | 1xchr3, t(3;?)(q27;?) | IHC/FISH |
| 2F2G2O (3) | 4n | 2xchr3, 2x t(3;?)(q27;?)† | (CD20/BCL6) | |||
| (BCL6/BCL6) | ||||||
| 4 | D | NK | 1F1G1O (18) | 2n | 1xchr.3, t(3;?)(q27;?) | IHC/FISH |
| 2F1G1O (3) | 3n | 2xchr.3, t(3;?)(q27;?) | (CD20/BCL6) | |||
| 2F2G2O (1) | 4n | 2xchr.3, 2x t(3;?)(q27;?) | ||||
| 5F1G1O (1) | 6n | 5xch.3, t(3;?)(q27;?) | ||||
| 6F2G2O (2) | 8n | 6xch.3, 2x t(3;?)(q27;?) | ||||
| 5 | D | NM | 1F1G1O (4) | 2n | 1xchr.3, t(3;?)(q27;?) | IHC/FISH |
| 2F1G1O (1) | 3n | 2xchr.3, t(3;?)(q27;?) | (CD20/BCL6) | |||
| 2F2G2O (6) | 4n | 2xchr.3, 2x t(3;?)(q27;?) | ||||
| 6 | D | NM | 2F1O (6) | 2n | 2xchr.3, der(?)t(3;?)(q27;?)† | ND |
| 13 | D | ND | 1F2G2O (1) | 3n | 1xchr.3, 2xt(3;?)(q27;?) | FICTION |
| 2F1G1O (4) | 3n | 2xchr.3, t(3;?)(q27;?) | (CD20 +BCL6) | |||
| 3F1G1O (2) | 4n | 3xchr.3, t(3;?)(q27;?) | ||||
| 14 | D | ND | 1F2G1O (6) | 3n | 1xchr.3, t(3;?)(q27;?), der(3) t(3;?)(q27;?) | FICTION |
| 2F4G2O (1) | 6n | 2xchr.3, 2x t(3;?)(q27;?), 2xder(3) | (CD20 + BCL6) | |||
| t(3;?)(q27;?) | ||||||
| 15(a) | D | ND | 2F1G1O (10) | 3n | 2xchr.3, t(3;?)(q27;?) | FICTION |
| (CD20 +BCL6) | ||||||
| 15(b) | R | ND | 2F1G (2) | 3n | 2xchr.3, der(3) t(3;?)(q27;?) | FICTION |
| 3F1G (6) | 4n | 3xchr.3, der(3) t(3;?)(q27;?) | (CD20 + BCL6) | |||
| 16 | D | ND | 1F1G1O (12) | 2n | 1xchr.3, t(3;?)(q27;?) | FICTION |
| 1F2G2O (6) | 3n | 1xchr.3, 2x t(3;?)(q27;?) | (CD20 + BCL6) | |||
| 2F1G1O (2) | 3n | 2xchr.3, t(3;?)(q27;?) | ||||
| 3F1G1O (1) | 4n | 3xchr.3, t(3;?)(q27;?) | ||||
| 6F2G2O (2) | 8n | 6xchr.3, 2xt(3;?)(q27;?) | ||||
| 17(a) | D | ND | 2F1G (7) | 3n | 2xchr.3, der(3)t(3;?)(q27;?) | ND |
| 17(b) | R | ND | 2F1G (8) | 3n | 2xchr.3, der(3)t(3;?)(q27;?) | ND |
| 3F1G (3) | 4n | 3xchr.3, der(3)t(3;?)(q27;?) | ||||
| Case (LN sample) . | Status . | Cytogenetics/3q27 abnormalities . | Interphase FISH patterns found with LSI BCL6 (no. of nuclei) . | Interpretations of FISH results . | Confirmation of BCL6-R . | |
|---|---|---|---|---|---|---|
| Ploidy* . | Status of 3q27/BCL6 . | |||||
| 1 | D | t(3;22)(q27;q11) | 1F1G1O (10) | 2n | 1xchr3, t(3;22)(q27;q11) | IHC/FISH |
| (BCL6/BCL6) | ||||||
| 2(a) | D | der(3)t(3;?;1)(q27;?;q21) | ND | ND | ||
| 2(b) | R | der(3)t(3;?;1)(q27;?;q21) | 2F4G2O (6) | 4n† | 2xchr.3, 2xder(3)t(3;7;3;1)(3pter_q27::7p12→7pter:: 3q27::7p12::1q21→1qter), 2xder(7)t(3;7)(q27;p12)† | ND |
| 3 | D | NK | 1F1G1O (3) | 2n | 1xchr3, t(3;?)(q27;?) | IHC/FISH |
| 2F2G2O (3) | 4n | 2xchr3, 2x t(3;?)(q27;?)† | (CD20/BCL6) | |||
| (BCL6/BCL6) | ||||||
| 4 | D | NK | 1F1G1O (18) | 2n | 1xchr.3, t(3;?)(q27;?) | IHC/FISH |
| 2F1G1O (3) | 3n | 2xchr.3, t(3;?)(q27;?) | (CD20/BCL6) | |||
| 2F2G2O (1) | 4n | 2xchr.3, 2x t(3;?)(q27;?) | ||||
| 5F1G1O (1) | 6n | 5xch.3, t(3;?)(q27;?) | ||||
| 6F2G2O (2) | 8n | 6xch.3, 2x t(3;?)(q27;?) | ||||
| 5 | D | NM | 1F1G1O (4) | 2n | 1xchr.3, t(3;?)(q27;?) | IHC/FISH |
| 2F1G1O (1) | 3n | 2xchr.3, t(3;?)(q27;?) | (CD20/BCL6) | |||
| 2F2G2O (6) | 4n | 2xchr.3, 2x t(3;?)(q27;?) | ||||
| 6 | D | NM | 2F1O (6) | 2n | 2xchr.3, der(?)t(3;?)(q27;?)† | ND |
| 13 | D | ND | 1F2G2O (1) | 3n | 1xchr.3, 2xt(3;?)(q27;?) | FICTION |
| 2F1G1O (4) | 3n | 2xchr.3, t(3;?)(q27;?) | (CD20 +BCL6) | |||
| 3F1G1O (2) | 4n | 3xchr.3, t(3;?)(q27;?) | ||||
| 14 | D | ND | 1F2G1O (6) | 3n | 1xchr.3, t(3;?)(q27;?), der(3) t(3;?)(q27;?) | FICTION |
| 2F4G2O (1) | 6n | 2xchr.3, 2x t(3;?)(q27;?), 2xder(3) | (CD20 + BCL6) | |||
| t(3;?)(q27;?) | ||||||
| 15(a) | D | ND | 2F1G1O (10) | 3n | 2xchr.3, t(3;?)(q27;?) | FICTION |
| (CD20 +BCL6) | ||||||
| 15(b) | R | ND | 2F1G (2) | 3n | 2xchr.3, der(3) t(3;?)(q27;?) | FICTION |
| 3F1G (6) | 4n | 3xchr.3, der(3) t(3;?)(q27;?) | (CD20 + BCL6) | |||
| 16 | D | ND | 1F1G1O (12) | 2n | 1xchr.3, t(3;?)(q27;?) | FICTION |
| 1F2G2O (6) | 3n | 1xchr.3, 2x t(3;?)(q27;?) | (CD20 + BCL6) | |||
| 2F1G1O (2) | 3n | 2xchr.3, t(3;?)(q27;?) | ||||
| 3F1G1O (1) | 4n | 3xchr.3, t(3;?)(q27;?) | ||||
| 6F2G2O (2) | 8n | 6xchr.3, 2xt(3;?)(q27;?) | ||||
| 17(a) | D | ND | 2F1G (7) | 3n | 2xchr.3, der(3)t(3;?)(q27;?) | ND |
| 17(b) | R | ND | 2F1G (8) | 3n | 2xchr.3, der(3)t(3;?)(q27;?) | ND |
| 3F1G (3) | 4n | 3xchr.3, der(3)t(3;?)(q27;?) | ||||
LN indicates lymph node; D, at diagnosis; R, at relapse; NK, normal karyotype; NM, no mitosis; ND, not done; F, fused signal; G, green signal; and O, orange signal.
2n indicates diploid; 3n, triploid; 4n, tetraploid; 8n, octaploid.
Interpretation based on further FISH studies (I.W. et al, in preparation).