Nucleoside transporter expression in normal human leukocytes and human cell lines
| . | ENT1 . | ENT2 . | CNT1 . | CNT2 . | CNT3 . |
|---|---|---|---|---|---|
| Raji | +++ | ++ | − | ++ | − |
| Daudi | +++ | ++ | − | + | − |
| Jurkat | ++ | ++ | − | ++ | − |
| Granta | +++ | ++ | − | + | − |
| NCEB | +++ | ++ | − | + | + |
| JVM-2 | +++ | ++ | − | + | + |
| DOHH | +++ | ++ | − | + | − |
| NB4 | +++ | ++ | − | ++ | ++ |
| Mononuclear cells | +++ | ++ | − | +++ | + |
| Granulocytes | ++ | ++ | − | +++ | + |
| SW480 | +++ | +++ | + | + | − |
| Kidney | ND | ND | ++ | ND | ++ |
| . | ENT1 . | ENT2 . | CNT1 . | CNT2 . | CNT3 . |
|---|---|---|---|---|---|
| Raji | +++ | ++ | − | ++ | − |
| Daudi | +++ | ++ | − | + | − |
| Jurkat | ++ | ++ | − | ++ | − |
| Granta | +++ | ++ | − | + | − |
| NCEB | +++ | ++ | − | + | + |
| JVM-2 | +++ | ++ | − | + | + |
| DOHH | +++ | ++ | − | + | − |
| NB4 | +++ | ++ | − | ++ | ++ |
| Mononuclear cells | +++ | ++ | − | +++ | + |
| Granulocytes | ++ | ++ | − | +++ | + |
| SW480 | +++ | +++ | + | + | − |
| Kidney | ND | ND | ++ | ND | ++ |
Expression of the 5 nucleoside transporters in normal human leukocytes and human cell lines were analyzed by quantitative RT-PCR. The colon-derived cell line SW480 and kidney cDNA were used as positive control of CNT1 mRNA expression. Normalized CTvalues (ratio between each nucleoside transporter and the endogenous control) have been replaced by symbols that show the degree of expression: +, low expression (r < 1); ++, moderate expression (r = 1–1.3); +++, high expression (r > 1.3); −, no expression.
ND indicates not determined.