Oligonucleotide probes and primers used for quantitative RT-PCR analysis of nucleoside transporter gene expression
| . | Sense primer (5′-3′) . | Antisense primer (5′-3′) . | Probe (FAM-5′-3′-TAMRA) . |
|---|---|---|---|
| hCNT1 | TGA TTT CTT GGA AAG CCT GGA | CTG CTC CTG ATC TCT GCG G | AAG GCC AGC TCC CTA GGA GTG ACT TGA G |
| hCNT2 | AAG TAG AGC CTG AGG GAA GCA A | GCC CAG TCC ATC CCC C | AGG ACT GAC GCA CAA GGA CAC AGC C |
| hCNT3 | GAG CTG TGC AAA GCA GGG A | TGG AGA ATC CTG CTC AAC TGT G | CAC ACA AAC ACC AAA CAG GAT GAA GAA CAG G |
| hENT1 | GCA AAG GAG AGG AGC CAA GA | TTC ATT GGT GGG CTG AGA GTT | CAG GCA AAG AGG AAT CTG GAG TTT CAG TCT C |
| hENT2 | CCC TGG ATC TTG ACC TGG AG | GGT TTT CCT GGC TTC TGG G | AGG AGC CGG AAT CAG AGC CAG ATG A |
| . | Sense primer (5′-3′) . | Antisense primer (5′-3′) . | Probe (FAM-5′-3′-TAMRA) . |
|---|---|---|---|
| hCNT1 | TGA TTT CTT GGA AAG CCT GGA | CTG CTC CTG ATC TCT GCG G | AAG GCC AGC TCC CTA GGA GTG ACT TGA G |
| hCNT2 | AAG TAG AGC CTG AGG GAA GCA A | GCC CAG TCC ATC CCC C | AGG ACT GAC GCA CAA GGA CAC AGC C |
| hCNT3 | GAG CTG TGC AAA GCA GGG A | TGG AGA ATC CTG CTC AAC TGT G | CAC ACA AAC ACC AAA CAG GAT GAA GAA CAG G |
| hENT1 | GCA AAG GAG AGG AGC CAA GA | TTC ATT GGT GGG CTG AGA GTT | CAG GCA AAG AGG AAT CTG GAG TTT CAG TCT C |
| hENT2 | CCC TGG ATC TTG ACC TGG AG | GGT TTT CCT GGC TTC TGG G | AGG AGC CGG AAT CAG AGC CAG ATG A |