Expression of hematopoietic and endothelial surface markers in wild-type and SCL−/− EBs during differentiation in vitro
| Marker . | Positive cells, % . | ||||
|---|---|---|---|---|---|
| d2.75 . | d3.75 . | d4.75 . | d5.75 . | d6.75 . | |
| Wild-type EBs | |||||
| Flk1 | 3.2 ± 1.6 | 39.1 ± 2.4 | 24.7 ± 1.7 | 26.1 ± 1.8 | 25.8 ± 2.1 |
| c-kit | 41.3 ± 5.6 | 43.4 ± 3.7 | 53.1 ± 5.2 | 51.5 ± 2.6 | 40.3 ± 3.6 |
| α4-integrin | — | 2.5 ± 0.5 | 20.2 ± 0.6 | 42.9 ± 5.3 | 56.9 ± 6.2 |
| Endoglin | — | 25.1 ± 7 | 24.3 ± 9.9 | 15.0 ± 1 | 22.5 ± 1.2 |
| PECAM | — | 2 ± 0.8 | 14.3 ± 6.3 | 22.4 ± 7.8 | 24.4 ± 1.7 |
| MECA32 | — | 8.4 ± 3.2 | 8.1 ± 3.6 | 18.1 ± 2.7 | 28 ± 1.8 |
| VE-cadherin | — | — | — | 3.9 ± 1 | 4.9 ± 1 |
| CD34 | — | — | — | 1.7 ± 0.9 | 7.6 ± 1.4 |
| CD41 | — | — | 1.3 ± 0.7 | 8 ± 2.0 | 17.7 ± 3.4 |
| CD45 | — | — | — | 0.8 ± 0.5 | 1.9 ± 0.5 |
| Ter119 | — | — | — | — | 1.6 ± 0.2 |
| SCL−/−EBs | |||||
| Flk1 | 2.4 ± 1.3 | 53.7 ± 5.2 | 22.2 ± 2.9 | 29.6 ± 6.9 | 33.9 ± 1.4 |
| c-kit | 47.8 ± 3.1 | 35.2 ± 2 | 68.9 ± 5.5 | 66.3 ± 8.2 | 62.4 ± 2.8 |
| α4-integrin | — | 2 ± 0.5 | 42.6 ± 4.4 | 58.8 ± 6.2 | 65.5 ± 3.1 |
| Endoglin | — | 37.1 ± 5.6 | 35.6 ± 2.8 | 23.0 ± 1 | 36.7 ± 6.6 |
| PECAM | — | 2.5 ± 0.7 | 14.7 ± 6.1 | 22.3 ± 7.3 | 18.1 ± 2.2 |
| MECA32 | — | 7.2 ± 0.4 | 14.8 ± 6.1 | 21.2 ± 0.1 | 27.2 ± 1.9 |
| VE-cadherin | — | — | — | 2.8 ± 1.1 | 3.9 ± 0.1 |
| CD34 | — | — | — | — | 5.6 ± 1.5 |
| CD41 | — | — | — | — | — |
| CD45 | — | — | — | — | — |
| Ter119 | — | — | — | — | — |
| Marker . | Positive cells, % . | ||||
|---|---|---|---|---|---|
| d2.75 . | d3.75 . | d4.75 . | d5.75 . | d6.75 . | |
| Wild-type EBs | |||||
| Flk1 | 3.2 ± 1.6 | 39.1 ± 2.4 | 24.7 ± 1.7 | 26.1 ± 1.8 | 25.8 ± 2.1 |
| c-kit | 41.3 ± 5.6 | 43.4 ± 3.7 | 53.1 ± 5.2 | 51.5 ± 2.6 | 40.3 ± 3.6 |
| α4-integrin | — | 2.5 ± 0.5 | 20.2 ± 0.6 | 42.9 ± 5.3 | 56.9 ± 6.2 |
| Endoglin | — | 25.1 ± 7 | 24.3 ± 9.9 | 15.0 ± 1 | 22.5 ± 1.2 |
| PECAM | — | 2 ± 0.8 | 14.3 ± 6.3 | 22.4 ± 7.8 | 24.4 ± 1.7 |
| MECA32 | — | 8.4 ± 3.2 | 8.1 ± 3.6 | 18.1 ± 2.7 | 28 ± 1.8 |
| VE-cadherin | — | — | — | 3.9 ± 1 | 4.9 ± 1 |
| CD34 | — | — | — | 1.7 ± 0.9 | 7.6 ± 1.4 |
| CD41 | — | — | 1.3 ± 0.7 | 8 ± 2.0 | 17.7 ± 3.4 |
| CD45 | — | — | — | 0.8 ± 0.5 | 1.9 ± 0.5 |
| Ter119 | — | — | — | — | 1.6 ± 0.2 |
| SCL−/−EBs | |||||
| Flk1 | 2.4 ± 1.3 | 53.7 ± 5.2 | 22.2 ± 2.9 | 29.6 ± 6.9 | 33.9 ± 1.4 |
| c-kit | 47.8 ± 3.1 | 35.2 ± 2 | 68.9 ± 5.5 | 66.3 ± 8.2 | 62.4 ± 2.8 |
| α4-integrin | — | 2 ± 0.5 | 42.6 ± 4.4 | 58.8 ± 6.2 | 65.5 ± 3.1 |
| Endoglin | — | 37.1 ± 5.6 | 35.6 ± 2.8 | 23.0 ± 1 | 36.7 ± 6.6 |
| PECAM | — | 2.5 ± 0.7 | 14.7 ± 6.1 | 22.3 ± 7.3 | 18.1 ± 2.2 |
| MECA32 | — | 7.2 ± 0.4 | 14.8 ± 6.1 | 21.2 ± 0.1 | 27.2 ± 1.9 |
| VE-cadherin | — | — | — | 2.8 ± 1.1 | 3.9 ± 0.1 |
| CD34 | — | — | — | — | 5.6 ± 1.5 |
| CD41 | — | — | — | — | — |
| CD45 | — | — | — | — | — |
| Ter119 | — | — | — | — | — |
— indicates negative staining.