Semiquantitative analyses of P2Y1 in the membrane systems of platelets before and after stimulation with ADP or desensitization with ADPβS
| Platelets . | Gold particles per platelet section, no.* . | Mean surface area per platelet section, μm2 . | ||
|---|---|---|---|---|
| Surface . | Internal membranes . | Total . | ||
| Controls | ||||
| Unstimulated | 9.48 ± 7.31 | 53.30 ± 25.43 | 62.77 ± 29.54 | 2.36 ± 0.92 |
| ADP stimulated | 17.37 ± 12.91 | 62.8 ± 31.10 | 80.20 ± 37.34 | 2.77 ± 0.91 |
| Desensitized | 9.49 ± 7.38 | 48.81 ± .14 | 58.30 ± 31.96 | 2.22 ± 1.1 |
| Patient ML | ||||
| Unstimulated | 11.22 ± 7.35 | 44.59 ± 16.30 | 55.81 ± 19.39 | 2.58 ± 0.93 |
| ADP stimulated | 14.85 ± 11.13 | 59.02 ± 32.36 | 73.87 ± 40.07 | 2.8 ± 1.12 |
| Platelets . | Gold particles per platelet section, no.* . | Mean surface area per platelet section, μm2 . | ||
|---|---|---|---|---|
| Surface . | Internal membranes . | Total . | ||
| Controls | ||||
| Unstimulated | 9.48 ± 7.31 | 53.30 ± 25.43 | 62.77 ± 29.54 | 2.36 ± 0.92 |
| ADP stimulated | 17.37 ± 12.91 | 62.8 ± 31.10 | 80.20 ± 37.34 | 2.77 ± 0.91 |
| Desensitized | 9.49 ± 7.38 | 48.81 ± .14 | 58.30 ± 31.96 | 2.22 ± 1.1 |
| Patient ML | ||||
| Unstimulated | 11.22 ± 7.35 | 44.59 ± 16.30 | 55.81 ± 19.39 | 2.58 ± 0.93 |
| ADP stimulated | 14.85 ± 11.13 | 59.02 ± 32.36 | 73.87 ± 40.07 | 2.8 ± 1.12 |
Immunogold labeling using a MoAb to the amino terminal of P2Y1 was as described in “Materials and methods.” Gold particles were counted visually on the sections. Control values for irrelevant antibody were fewer than 3 particles per 100 sections. Surface area was calculated by computer analysis. Results are ± SD.