Oligonucleotide primers
| Primer . | Gene and primer direction . | Sequence (5′ - 3′) . | Nucleotide Position . |
|---|---|---|---|
| S1 | Oligo (dT) | 5′-Bn-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG- (dT)24-3′ | |
| ▸ | — | ||
| Cassette A | SAGE | 5′-TTTGGATTTGCTGGTCGAGTACAACTAGGCTTAATCCGACATG-3′ | — |
| 3′-*CCTAAACGACCAGCTCATGTTGATCCGAATAAGGCTp-5′ | |||
| Cassette B | SAGE | 5′-pTTCATGGCGGAGACGTCCGCCACTAGTGTCGCAACTGACTA*-3′ | — |
| 3′-NNAAGTACCGCCTCTGCAGGCGGTGATCACAGCGTTGACTGAT-5′ | |||
| ◂ | |||
| S2 | SAGE | 5′-Bn-GGATTTGCTGGTCGAGTACA-3′ | — |
| S3 | SAGE | 5′-Bn-TAGTCAGGTGCGACACTAGTGGC-3′ | — |
| GP4 | Glycoprotein IIB [F] | 5′-AGGGCTTTGAGAGACTCATCTGTA-3′ | 2094-2117 |
| GP5 | Glycoprotein IIB [R] | 5′-ACAATCTTGCTGTTTGGATTCTG-3′ | 2301-2279 |
| GP6 | Glycoprotein IIIA [F] | 5′-TATAAAGAGGCCACGTCTACCTTC-3′ | 2335-2358 |
| GP7 | Glycoprotein IIIA [R] | 5′-CACTTCCACATACTGACATTCTCC-3′ | 2532-2509 |
| PAR18 | PAR1 [F] | 5′-AATGTCAGTTCTGATATGGAAGCA-3′ | 2585-2608 |
| PAR19 | PAR1 [R] | 5′-CCCAAATGTTCAAACTTCTTTAGC-3′ | 2776-2753 |
| SR8 | 16S rRNA [F] | 5′-TGCAAAGGTAGCATAATCACTTGT-3′ | 2586-2609 |
| SR9 | 16S rRNA [R] | 5′-GTTTAGGACCTGTGGGTTTGTTAG-3′ | 2785-2762 |
| NADH10 | NADH2 [F] | 5′-CTAGCCCCCATCTCAAATCATATAC-3′ | 4875-4898 |
| NADH11 | NADH2 [R] | 5′-AATGGTTATGTTAGGGTTGTACGG-3′ | 5075-5052 |
| THYM12 | Thymosin β4 [F] | 5′-AAGACAGAGACGCAAGAGAAAAAT-3′ | 135-158 |
| THYM13 | Thymosin β4 [R] | 5′-GCAGCACAGTCATTTAAACTTGAT-3′ | 336-313 |
| CLUS14 | Clusterin [F] | 5′-CCAACAGAATTCATACGAGAAGG-3′ | 1006-1028 |
| CLUS15 | Clusterin [R] | 5′-CGTTATATTTCCTGGTCAACCTCT-3′ | 1222-1199 |
| NRG16 | Neurogranin [F] | 5′-GCCCTTTTAGTTAGTTCTGCAGTC-3′ | 1351-1374 |
| NRG17 | Neurogranin [R] | 5′-TTTTCTTTAAGTGAGTGTGCTTGG-3′ | 1567-1544 |
| TCR18 | T-cell receptor β-chain [F] | 5′-CCACAACTATGTTTTGGTATCGT-3′ | 131-153 |
| TCR19 | T-cell receptor β-chain [R] | 5′-CTAGCACTGCAGATGTAGAAGCT-3′ | 332-310 |
| CD4520 | CD45 [F] | 5′-GCTCAGAATGGACAAGTA-3′ | 3771-3788 |
| CD4521 | CD45 [R] | 5′-CACACCCATACACACATACA-3′ | 4280-4261 |
| Primer . | Gene and primer direction . | Sequence (5′ - 3′) . | Nucleotide Position . |
|---|---|---|---|
| S1 | Oligo (dT) | 5′-Bn-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG- (dT)24-3′ | |
| ▸ | — | ||
| Cassette A | SAGE | 5′-TTTGGATTTGCTGGTCGAGTACAACTAGGCTTAATCCGACATG-3′ | — |
| 3′-*CCTAAACGACCAGCTCATGTTGATCCGAATAAGGCTp-5′ | |||
| Cassette B | SAGE | 5′-pTTCATGGCGGAGACGTCCGCCACTAGTGTCGCAACTGACTA*-3′ | — |
| 3′-NNAAGTACCGCCTCTGCAGGCGGTGATCACAGCGTTGACTGAT-5′ | |||
| ◂ | |||
| S2 | SAGE | 5′-Bn-GGATTTGCTGGTCGAGTACA-3′ | — |
| S3 | SAGE | 5′-Bn-TAGTCAGGTGCGACACTAGTGGC-3′ | — |
| GP4 | Glycoprotein IIB [F] | 5′-AGGGCTTTGAGAGACTCATCTGTA-3′ | 2094-2117 |
| GP5 | Glycoprotein IIB [R] | 5′-ACAATCTTGCTGTTTGGATTCTG-3′ | 2301-2279 |
| GP6 | Glycoprotein IIIA [F] | 5′-TATAAAGAGGCCACGTCTACCTTC-3′ | 2335-2358 |
| GP7 | Glycoprotein IIIA [R] | 5′-CACTTCCACATACTGACATTCTCC-3′ | 2532-2509 |
| PAR18 | PAR1 [F] | 5′-AATGTCAGTTCTGATATGGAAGCA-3′ | 2585-2608 |
| PAR19 | PAR1 [R] | 5′-CCCAAATGTTCAAACTTCTTTAGC-3′ | 2776-2753 |
| SR8 | 16S rRNA [F] | 5′-TGCAAAGGTAGCATAATCACTTGT-3′ | 2586-2609 |
| SR9 | 16S rRNA [R] | 5′-GTTTAGGACCTGTGGGTTTGTTAG-3′ | 2785-2762 |
| NADH10 | NADH2 [F] | 5′-CTAGCCCCCATCTCAAATCATATAC-3′ | 4875-4898 |
| NADH11 | NADH2 [R] | 5′-AATGGTTATGTTAGGGTTGTACGG-3′ | 5075-5052 |
| THYM12 | Thymosin β4 [F] | 5′-AAGACAGAGACGCAAGAGAAAAAT-3′ | 135-158 |
| THYM13 | Thymosin β4 [R] | 5′-GCAGCACAGTCATTTAAACTTGAT-3′ | 336-313 |
| CLUS14 | Clusterin [F] | 5′-CCAACAGAATTCATACGAGAAGG-3′ | 1006-1028 |
| CLUS15 | Clusterin [R] | 5′-CGTTATATTTCCTGGTCAACCTCT-3′ | 1222-1199 |
| NRG16 | Neurogranin [F] | 5′-GCCCTTTTAGTTAGTTCTGCAGTC-3′ | 1351-1374 |
| NRG17 | Neurogranin [R] | 5′-TTTTCTTTAAGTGAGTGTGCTTGG-3′ | 1567-1544 |
| TCR18 | T-cell receptor β-chain [F] | 5′-CCACAACTATGTTTTGGTATCGT-3′ | 131-153 |
| TCR19 | T-cell receptor β-chain [R] | 5′-CTAGCACTGCAGATGTAGAAGCT-3′ | 332-310 |
| CD4520 | CD45 [F] | 5′-GCTCAGAATGGACAAGTA-3′ | 3771-3788 |
| CD4521 | CD45 [R] | 5′-CACACCCATACACACATACA-3′ | 4280-4261 |
[F] indicates forward (sense) strand; [R], reverse (antisense) strand; Bn, biotin; p, a phosphorylated 5′ end (cassettes A and B); underlining, NlaIII sites in cassettes A and B; arrows, corresponding sequence for S2 and S3 within cassettes A and B, respectively; bold, the MmeI site; and N, A, C, T, or G, nucleotide position based on the following accession numbers: glycoprotein IIB (J02764), glycoprotein IIIA (M35999), PAR1 (M62424), 16S rRNA and NADH2 (NC_001807), thymosin β4 (M17733), clusterin (M25915), neurogranin (X99076), TCR β-chain (AF043182), CD45 (Y00638).
Indicates an amino-modified 3′ end in both cassettes; —, not applicable.