“Direct” and “indirect” target genes of C/EBPα in primary human hematopoietic cells
| Accession no. . | Gene title . | Fold change . | |||||
|---|---|---|---|---|---|---|---|
| Exp no. 1 . | Exp no. 2 . | Exp no. 3 . | Average . | Exp 2 h β-ES . | Exp 8 h β-ES + CHX . | ||
| AI126134 | Calgranulin A, MRP-8 | 3.73 | 3.25 | 4.00 | 3.49 | NC | 1.90 |
| X97324 | Adipophilin | 3.25 | 4.29 | 3.25 | 3.77 | 2.00 | 2.10 |
| X77956 | Id1 | 3.48 | 3.73 | 3.03 | 3.61 | 2.10 | 1.50 |
| W72424 | Calgranulin B | 3.25 | 2.14 | 2.46 | 2.70 | NC | NC |
| X52015 | IL-1 receptor antagonist | 2.14 | 1.87 | 2.46 | 2.00 | 2.46 | NC |
| X69910 | p63 transmembrane protein | 1.74 | 1.87 | 2.83 | 1.80 | 1.52 | 1.52 |
| U30255 | hPGDH | 1.52 | 1.62 | 1.87 | 1.57 | NC | NC |
| AJ005259 | EDF-1 | −1.52 | −1.52 | −1.74 | −1.52 | NC | NC |
| D32257 | GTF3A | −1.62 | −1.62 | −1.74 | −1.62 | NC | NC |
| D00265 | Cytochrome C | −1.52 | −1.87 | −1.62 | −1.69 | NC | NC |
| D25538 | KIAA0037 | −1.62 | −1.62 | −2.30 | −1.62 | NC | NC |
| U41387 | Gu protein | −1.62 | −2.46 | −1.52 | −2.04 | NC | NC |
| Z35278 | PEBP2aC1 | −2.30 | −1.74 | −2.30 | −2.02 | −1.60 | −1.50 |
| AL049432 | RAI17 | −2.64 | −2.30 | −1.74 | −2.47 | NC | NC |
| V00568 | c-myc oncogene | −2.83 | −2.00 | −1.87 | −2.42 | NC | NC |
| Accession no. . | Gene title . | Fold change . | |||||
|---|---|---|---|---|---|---|---|
| Exp no. 1 . | Exp no. 2 . | Exp no. 3 . | Average . | Exp 2 h β-ES . | Exp 8 h β-ES + CHX . | ||
| AI126134 | Calgranulin A, MRP-8 | 3.73 | 3.25 | 4.00 | 3.49 | NC | 1.90 |
| X97324 | Adipophilin | 3.25 | 4.29 | 3.25 | 3.77 | 2.00 | 2.10 |
| X77956 | Id1 | 3.48 | 3.73 | 3.03 | 3.61 | 2.10 | 1.50 |
| W72424 | Calgranulin B | 3.25 | 2.14 | 2.46 | 2.70 | NC | NC |
| X52015 | IL-1 receptor antagonist | 2.14 | 1.87 | 2.46 | 2.00 | 2.46 | NC |
| X69910 | p63 transmembrane protein | 1.74 | 1.87 | 2.83 | 1.80 | 1.52 | 1.52 |
| U30255 | hPGDH | 1.52 | 1.62 | 1.87 | 1.57 | NC | NC |
| AJ005259 | EDF-1 | −1.52 | −1.52 | −1.74 | −1.52 | NC | NC |
| D32257 | GTF3A | −1.62 | −1.62 | −1.74 | −1.62 | NC | NC |
| D00265 | Cytochrome C | −1.52 | −1.87 | −1.62 | −1.69 | NC | NC |
| D25538 | KIAA0037 | −1.62 | −1.62 | −2.30 | −1.62 | NC | NC |
| U41387 | Gu protein | −1.62 | −2.46 | −1.52 | −2.04 | NC | NC |
| Z35278 | PEBP2aC1 | −2.30 | −1.74 | −2.30 | −2.02 | −1.60 | −1.50 |
| AL049432 | RAI17 | −2.64 | −2.30 | −1.74 | −2.47 | NC | NC |
| V00568 | c-myc oncogene | −2.83 | −2.00 | −1.87 | −2.42 | NC | NC |
Human hematopoietic cells transduced with MIGR1 or MIGR1 C/EBPα-ER were sorted for the expression of GFP and grown in liquid culture in the presence of human cytokines and β-estradiol for 8 hours. Transcriptional profiles were analyzed using Affymetrix HU95A oligonucleotide arrays. Data are from 3 independent experiments (Exp) using the following criteria for differentially expressed genes: (1) indicated as present by the “absolute call,” (2) indicated as induced or decreased by the “difference call,” and (3) at least a 1.5-fold change in expression level with P≤.005 The estrogen receptor was indicated as up-regulated, because of the use of the C/EBPα-ER fusion protein, but it is not listed as a “target gene.” Fold change (“Average”) indicates the average change in gene expression of the 3 independent experiments. To identify “direct” target genes of C/EBPα, transcriptional profiles were analyzed 2 hours after the addition of β-estradiol (“Exp 2 h β-ES”) or 8 hours after the addition of β-estradiol in the presence of cycloheximide (“Exp 8 h β-ES+CHX”). The fold change in expression of the C/EBPα target genes is listed in the indicated columns.
NC indicates < 1.5 fold change or P > .005.