Primers used for extrachromosomal VDJ recombination assays and for generation of the wt Artemis expression construct
| Primer . | Sequence . |
|---|---|
| NV09F | 5′-CGTCTTCAAGAATTGTCGATGAATTCC-3′ |
| DG147 | 5′-TACATTGAGCAACTGACTGAAATGCC-3′ |
| NV08F | 5′-AACTAGTTAACTATACGCAGCTTGGC-3′ |
| FM30 | 5′-CTCCATTTTAGCTTCCTTAGCTCCTG-3′ |
| DG238 | 5′-TCCGCTCGAGGCGGCGCTATGAGTTCTTTCG-3′ |
| DG239 | 5′-TCCGACGCGTTGGTATCTAAGAGTGAGCATTTTCTT-3′ |
| Primer . | Sequence . |
|---|---|
| NV09F | 5′-CGTCTTCAAGAATTGTCGATGAATTCC-3′ |
| DG147 | 5′-TACATTGAGCAACTGACTGAAATGCC-3′ |
| NV08F | 5′-AACTAGTTAACTATACGCAGCTTGGC-3′ |
| FM30 | 5′-CTCCATTTTAGCTTCCTTAGCTCCTG-3′ |
| DG238 | 5′-TCCGCTCGAGGCGGCGCTATGAGTTCTTTCG-3′ |
| DG239 | 5′-TCCGACGCGTTGGTATCTAAGAGTGAGCATTTTCTT-3′ |