Influence of isotype-selective adenosine receptor agonist on cytokine release by mDCs
| DC . | Control . | CHA . | DPMA* . | IB-MECA . |
|---|---|---|---|---|
| IL-12 | 6327 ± 845 | 6156 ± 932 | 1563 ± 328 | 6547 ± 924 |
| TNF-α | 8552 ± 472 | 8156 ± 672 | 2100 ± 567 | 8295 ± 781 |
| IL-10 | 621 ± 156 | 576 ± 145 | 1872 ± 276 | 645 ± 187 |
| DC . | Control . | CHA . | DPMA* . | IB-MECA . |
|---|---|---|---|---|
| IL-12 | 6327 ± 845 | 6156 ± 932 | 1563 ± 328 | 6547 ± 924 |
| TNF-α | 8552 ± 472 | 8156 ± 672 | 2100 ± 567 | 8295 ± 781 |
| IL-10 | 621 ± 156 | 576 ± 145 | 1872 ± 276 | 645 ± 187 |
DCs were incubated with LPS in the absence or the presence of 10−6 M CHA (A1 receptor agonist), 10−6 M DPMA (A2 receptor agonist), and 10−6 M IB-MECA (A3 receptor agonist) for 48 hours. The release of IL-12, TNF-α, and IL-10 was quantified in the supernatant. Data are given as mean pg/mL/0.2 × 106 cells ± SEM (n = 3). Differences between cells stimulated with DPMA or control medium:P < .01 (*); Tukey multiple comparison test.