Influence of adenosine on cell-surface expression of membrane molecules on DCs
| DC . | Treatment . | |||
|---|---|---|---|---|
| None . | Adenosine . | LPS . | LPS/adenosine . | |
| CD86 | 42.2 ± 5.6 | 67.5 ± 4.7*,† | 388.9 ± 40.8 | 397.4 ± 28.8 |
| CD83 | 25.0 ± 6.4 | 25.2 ± 1.5 | 70.5 ± 6.0 | 77.0 ± 5.8 |
| CD80 | 34.0 ± 8.0 | 62.6 ± 6.7*,† | 161.2 ± 16.4 | 220.5 ± 18.5‡,1-153 |
| CD54 | 786.0 ± 100.1 | 850.2 ± 25.2 | 3055.3 ± 343.5 | 3905.2 ± 65.9‡,1-153 |
| MHC I | 180.2 ± 50.9 | 291.4 ± 19.5†,‡ | 390.1 ± 38.7 | 615.6 ± 57.01-153,1-155 |
| HLA-DR | 174.4 ± 60.8 | 290.6 ± 26.1†,1-155 | 425.4 ± 29.2 | 549.1 ± 26.7‡,1-153 |
| CD1a | 208.3 ± 14.8 | 86.8 ± 23.7†,1-155 | 62.2 ± 19.5 | 30.3 ± 13.2‡,1-153 |
| DC . | Treatment . | |||
|---|---|---|---|---|
| None . | Adenosine . | LPS . | LPS/adenosine . | |
| CD86 | 42.2 ± 5.6 | 67.5 ± 4.7*,† | 388.9 ± 40.8 | 397.4 ± 28.8 |
| CD83 | 25.0 ± 6.4 | 25.2 ± 1.5 | 70.5 ± 6.0 | 77.0 ± 5.8 |
| CD80 | 34.0 ± 8.0 | 62.6 ± 6.7*,† | 161.2 ± 16.4 | 220.5 ± 18.5‡,1-153 |
| CD54 | 786.0 ± 100.1 | 850.2 ± 25.2 | 3055.3 ± 343.5 | 3905.2 ± 65.9‡,1-153 |
| MHC I | 180.2 ± 50.9 | 291.4 ± 19.5†,‡ | 390.1 ± 38.7 | 615.6 ± 57.01-153,1-155 |
| HLA-DR | 174.4 ± 60.8 | 290.6 ± 26.1†,1-155 | 425.4 ± 29.2 | 549.1 ± 26.7‡,1-153 |
| CD1a | 208.3 ± 14.8 | 86.8 ± 23.7†,1-155 | 62.2 ± 19.5 | 30.3 ± 13.2‡,1-153 |
DCs were stimulated with and without 10−4 M adenosine in the presence or the absence of 3 μg/mL LPS for 48 hours. The expression of the indicated molecules or isotype controls was analyzed by flow cytometry. Data indicate the net MFI ± SEM (n = 3). Global differences between groups:P ≤ .0001 (ANOVA);
differences between cells stimulated with adenosine or control medium,
LPS/adenosine-stimulated versus LPS-treated cells:
P < .05.
P < .01.
P < .001; Tukey multiple comparison test.