Microhemodynamic parameters during the first 14 days of tumor growth
| Parameter . | Day . | |||
|---|---|---|---|---|
| 1 . | 3 . | 8 . | 14 . | |
| Vessel diameter, μm | ||||
| Controls | 20.2 ± 0.6 | 20.2 ± 0.8 | 21.0 ± 1.0 | 22.0 ± 0.7*,† |
| LLC-1 | 23.0 ± 2.0‡ | 17.7 ± 2.9 | 22.0 ± 3.0 | 22.0 ± 0.5 |
| BFS-1 | 21.5 ± 0.6 | 19.1 ± 0.4* | 21.1 ± 1.1 | 20.0 ± 0.8‡1-153 |
| RBC velocity, mm/s | ||||
| Controls | 0.32 ± 0.02 | 0.38 ± 0.07 | 0.35 ± 0.06 | 0.34 ± 0.05 |
| LLC-1 | 0.14 ± 0.04‡1-155 | 0.18 ± 0.05‡1-155 | 0.18 ± 0.09‡ | 0.28 ± 0.02*,† |
| BFS-1 | 0.25 ± 0.03 | 0.34 ± 0.04* | 0.25 ± 0.03† | 0.25 ± 0.02 |
| Wall shear rate, s−1 | ||||
| Controls | 122.0 ± 8.3 | 142.3 ± 32.3 | 134.4 ± 29.5 | 112.5 ± 19.0 |
| LLC-1 | 57.9 ± 12.5‡1-155 | 73.9 ± 25.8‡1-155 | 66.7 ± 24.4‡ | 98.5 ± 8.3*,† |
| BFS-1 | 86.6 ± 37.3 | 142.5 ± 20.2* | 94.3 ± 15.3† | 93.8 ± 9.3 |
| Parameter . | Day . | |||
|---|---|---|---|---|
| 1 . | 3 . | 8 . | 14 . | |
| Vessel diameter, μm | ||||
| Controls | 20.2 ± 0.6 | 20.2 ± 0.8 | 21.0 ± 1.0 | 22.0 ± 0.7*,† |
| LLC-1 | 23.0 ± 2.0‡ | 17.7 ± 2.9 | 22.0 ± 3.0 | 22.0 ± 0.5 |
| BFS-1 | 21.5 ± 0.6 | 19.1 ± 0.4* | 21.1 ± 1.1 | 20.0 ± 0.8‡1-153 |
| RBC velocity, mm/s | ||||
| Controls | 0.32 ± 0.02 | 0.38 ± 0.07 | 0.35 ± 0.06 | 0.34 ± 0.05 |
| LLC-1 | 0.14 ± 0.04‡1-155 | 0.18 ± 0.05‡1-155 | 0.18 ± 0.09‡ | 0.28 ± 0.02*,† |
| BFS-1 | 0.25 ± 0.03 | 0.34 ± 0.04* | 0.25 ± 0.03† | 0.25 ± 0.02 |
| Wall shear rate, s−1 | ||||
| Controls | 122.0 ± 8.3 | 142.3 ± 32.3 | 134.4 ± 29.5 | 112.5 ± 19.0 |
| LLC-1 | 57.9 ± 12.5‡1-155 | 73.9 ± 25.8‡1-155 | 66.7 ± 24.4‡ | 98.5 ± 8.3*,† |
| BFS-1 | 86.6 ± 37.3 | 142.5 ± 20.2* | 94.3 ± 15.3† | 93.8 ± 9.3 |
At the first day after tumor cell implantation, pre-existing venules on the edge of implanted tumor cells were included in the intravital microscopic studies, whereas newly developed tumor microvessels were observed on the following days. Subcutaneous venules of tumor-free chamber preparations served as controls. Wall shear rate was calculated from vessel diameter and red blood cell (RBC) velocity of individual microvessels. Data are given as the median ± standard error of the median; n = 6 experimental animals per group. P values are according to the Kruskal-Wallis test.
P < .05 versus day 1.
P < .05 versus day 3.
P < .05 versus controls.
P < .05 versus LLC-1.
P < .05 versus BFS-1.