Antibodies to TGF-β interfere with the induction of monocytic differentiation by vitamin D3
| Treatment . | NSE+, %* . | NBT+, %* . |
|---|---|---|
| Control | 3 ± 1 | 5 ± 3 |
| Vit D3 | 66 ± 5 | 3 ± 2 |
| 1D11 | 0 | 0 |
| Vit D3/1D11 | 23 ± 4 | 1 ± 1 |
| Vit D3/isotype control | 61, 57 | 7, 5 |
| TGF-β | 16 ± 7 | 0 |
| TGF-β/1D11 | 1 ± 1 | 0 |
| Treatment . | NSE+, %* . | NBT+, %* . |
|---|---|---|
| Control | 3 ± 1 | 5 ± 3 |
| Vit D3 | 66 ± 5 | 3 ± 2 |
| 1D11 | 0 | 0 |
| Vit D3/1D11 | 23 ± 4 | 1 ± 1 |
| Vit D3/isotype control | 61, 57 | 7, 5 |
| TGF-β | 16 ± 7 | 0 |
| TGF-β/1D11 | 1 ± 1 | 0 |
Cells were cultured for 6 days in 5% serum plus the following additions as indicated: Vit D3, 100 nM, TGF-β, 5 ng/mL; 1D11, 30 μg/mL, IgG1κ (isotype control, 30 μg/mL). NSE and NBT were assayed as described in “Materials and methods.” Data are the averaged values of 3 separate experiments ± standard error (SE), with the exception of the isotype control, which was performed twice.
Five hundred cells were counted.