Increased adhesion of αIIbβ3-inhibited platelets to PMA-ECM by VIIa-mediated thrombin generation is dependent on the GPIb-vWF interaction and on thrombin binding to GPIb
| Additions . | Surface coverage, % ± SEM . |
|---|---|
| None | 7.4 ± 2.13-150 (n = 18) |
| + VIIa/X/II | 48.2 ± 2.9 (n = 18) |
| + VIIa/X/II + anti-GPIb | 6.7 ± 1.53-150 (n = 8) |
| + VIIa/X/II + anti-VWF | 2.5 ± 1.33-150 (n = 4) |
| + VIIa/X/II + LJIb-10 | 8.3 ± 1.13-150 (n = 7) |
| Additions . | Surface coverage, % ± SEM . |
|---|---|
| None | 7.4 ± 2.13-150 (n = 18) |
| + VIIa/X/II | 48.2 ± 2.9 (n = 18) |
| + VIIa/X/II + anti-GPIb | 6.7 ± 1.53-150 (n = 8) |
| + VIIa/X/II + anti-VWF | 2.5 ± 1.33-150 (n = 4) |
| + VIIa/X/II + LJIb-10 | 8.3 ± 1.13-150 (n = 7) |
Washed platelets and red cells were preincubated with the RGD-containing peptide dRGDW and perfused over PMA-ECM at a shear rate of 1600 s−1 for 5 minutes. Platelets were preincubated with antibodies against GPIb, VWF, or the thrombin-binding site on GPIb (LJIb-10) for 45 minutes at room temperature; n is the number of evaluated coverslips.
P < .001 versus addition of VIIa/X/II.