Tissue factor-independent thrombin generation on procoagulant surface enhances deposition of αIIbβ3-inhibited platelets to PMA-ECM under flow conditions
| Additions . | Surface coverage, % ± SEM . |
|---|---|
| None | 7.4 ± 2.1* (n = 18) |
| + VIIa/X/II | 48.2 ± 2.9 (n = 18) |
| + VIIa/X/II + hirudin | 6.7 ± 0.9* (n = 7) |
| + VIIa/X/II + annexin V | 10.1 ± 2.0* (n = 6) |
| + VIIa/X/II + antitissue factor | 58.8 ± 4.1 (n = 8) |
| Additions . | Surface coverage, % ± SEM . |
|---|---|
| None | 7.4 ± 2.1* (n = 18) |
| + VIIa/X/II | 48.2 ± 2.9 (n = 18) |
| + VIIa/X/II + hirudin | 6.7 ± 0.9* (n = 7) |
| + VIIa/X/II + annexin V | 10.1 ± 2.0* (n = 6) |
| + VIIa/X/II + antitissue factor | 58.8 ± 4.1 (n = 8) |
Washed platelets and red cells were preincubated with the RGD containing peptide dRGDW and perfused over PMA-ECM at a shear rate of 1600 s−1 for 5 minutes. Hirudin or annexin V was added to the perfusate right before perfusion. Both the platelet suspension and the surface were preincubated with the anti–tissue factor antibody for 45 minutes at room temperature; n is the number of evaluated coverslips.
P < .001 versus addition of VIIa/X/II.