Reduced CUL-4A expression in CUL-4A+/− mice results in reduced erythroid and multipotential progenitors
| Genotype . | No. of progenitors per femur ± SEM . | |||
|---|---|---|---|---|
| CFU-GEMMs . | BFU-Es . | CFU-GMs . | Total . | |
| CUL-4A+/− | 1600 ± 200* | 700 ± 200† | 39 000 ± 4200 | 43 000 ± 4400 |
| Wild-type | 5100 ± 400* | 3700 ± 500† | 42 100 ± 5000 | 50 800 ± 5800 |
| Genotype . | No. of progenitors per femur ± SEM . | |||
|---|---|---|---|---|
| CFU-GEMMs . | BFU-Es . | CFU-GMs . | Total . | |
| CUL-4A+/− | 1600 ± 200* | 700 ± 200† | 39 000 ± 4200 | 43 000 ± 4400 |
| Wild-type | 5100 ± 400* | 3700 ± 500† | 42 100 ± 5000 | 50 800 ± 5800 |
Bone marrow was isolated from the femurs of 5CUL-4A+/− and 6 wild-type littermates and cultured for 7 days. Burst-forming units–erythroid (BFU-Es), CFU-GEMMs, and colony-forming units–granulocyte-macrophage (CFU-GMs), as well as total colonies, were counted, and the number of progenitors per femur was calculated. Erythroid cells were identified by benzidine staining to confirm the distinction between mixed-lineage and CFU-GM colonies. These results are representative of 3 independent experiments.
P = .0001 by Student t test.
P = .001 by Student t test.