Plasma FVIII and VWF levels after adenovirus-mediated overexpression of RAP
| Genotype and plasma protein . | Adenovirus . | Levels before adenovirus injection, U/mL . | Levels at day 3 after adenovirus injection, U/mL . |
|---|---|---|---|
| C57BL/6J | |||
| FVIII | Ad-β-Gal | 0.9 (0.8-1.0) | 1.3 (0.8-2.1) |
| Ad-RAP | 0.9 (0.9-1.0) | 3.2 (2.4-4.2)* | |
| VWF | Ad-β-Gal | 0.7 (0.5-0.9) | 2.0 (1.2-3.3) |
| Ad-RAP | 0.7 (0.6-0.7) | 2.4 (2.3-2.5) | |
| MX1cre+LRPflox/flox | |||
| FVIII | Ad-β-Gal | 2.4 (1.9-2.9) | 1.9 (1.0-3.4) |
| Ad-RAP | 1.9 (1.2-3.1) | 3.9 (3.5-4.4)* | |
| VWF | Ad-β-Gal | 1.4 (1.1-1.8) | 2.8 (2.5-3.2) |
| Ad-RAP | 1.3 (0.8-2.1) | 4.5 (3.5-5.6)* |
| Genotype and plasma protein . | Adenovirus . | Levels before adenovirus injection, U/mL . | Levels at day 3 after adenovirus injection, U/mL . |
|---|---|---|---|
| C57BL/6J | |||
| FVIII | Ad-β-Gal | 0.9 (0.8-1.0) | 1.3 (0.8-2.1) |
| Ad-RAP | 0.9 (0.9-1.0) | 3.2 (2.4-4.2)* | |
| VWF | Ad-β-Gal | 0.7 (0.5-0.9) | 2.0 (1.2-3.3) |
| Ad-RAP | 0.7 (0.6-0.7) | 2.4 (2.3-2.5) | |
| MX1cre+LRPflox/flox | |||
| FVIII | Ad-β-Gal | 2.4 (1.9-2.9) | 1.9 (1.0-3.4) |
| Ad-RAP | 1.9 (1.2-3.1) | 3.9 (3.5-4.4)* | |
| VWF | Ad-β-Gal | 1.4 (1.1-1.8) | 2.8 (2.5-3.2) |
| Ad-RAP | 1.3 (0.8-2.1) | 4.5 (3.5-5.6)* |
Normal C57BL/6J and MX1cre+LRPflox/floxmice were injected 3 times intraperitoneally with 250 μg pI:pC at 2-day intervals. At 6 weeks after the last pI:pC injection, mice were injected intravenously with 2 × 109 PFUs of Ad-RAP or Ad-β-Gal. Before and 3 days after adenovirus injection, blood was drawn and plasma was analyzed for FVIII and VWF, as described in “Materials and methods.” Values represent geometric mean values and 68% confidence intervals of 4 animals per group.
P < .05, effect of Ad-RAP significantly different from control Ad-β-Gal by means of the Mann-WhitneyU test.