CLL cell viability is sustained when cocultured with autologous Plexin-B1+ BM stromal cells
| Day . | CLL cells alone . | + Autologous stromal cells* . | + Autologous stromal cells + sCD100 . |
|---|---|---|---|
| 1 | 58 (± 0.7) | 81 (± 10.6) | 72 (± 10.6) |
| 3 | 57 (± 1.4) | 82 (± 5.6) | 65† (± 6.3) |
| 4 | 56 (± 4.9) | 84 (± 4.9) | 60† (± 2.1) |
| Day . | CLL cells alone . | + Autologous stromal cells* . | + Autologous stromal cells + sCD100 . |
|---|---|---|---|
| 1 | 58 (± 0.7) | 81 (± 10.6) | 72 (± 10.6) |
| 3 | 57 (± 1.4) | 82 (± 5.6) | 65† (± 6.3) |
| 4 | 56 (± 4.9) | 84 (± 4.9) | 60† (± 2.1) |
Leukemic cells significantly sustained their viability when cocultured with autologous Plexin-B1+ BM stromal cells in comparison with CLL cells alone. (*P < .01.) The effect on viability was significantly abolished by soluble CD100 (stromal cells + sCD100; †P < .05). Viability was assessed by cytofluorograph analysis of Annexin V staining. The numbers express the percentage of viable CLL cells and represent the mean values ± standard deviations obtained in 2 independent experiments.