Expression of GFP in recipients of transduced leukemias
| . | Control . | hC/EBPα . | hC/EBPɛ* . | rC/EBPα-WT† . | rC/EBPα-ΔuORF† . |
|---|---|---|---|---|---|
| PML-RARα leukemia | 67 ± 16 | 60 ± 8 | 4 ± 2 | NA | NA |
| no. 1111, 50 000 cells | (2 of 5) | (4 of 5) | (2 of 4) | ||
| PML-RARα leukemia | 88 ± 4 | 72 ± 9 | ‡0.2, 70, 80 | NA | NA |
| no. 1111, 25 000 cells | (3 of 4) | (4 of 5) | (3 of 5) | ||
| PML-RARα leukemia | 56 ± 12 | 59 ± 5 | 0.6 ± 0.3 | NA | NA |
| no. 935, 50 000 cells | (5 of 6) | (5 of 6) | (3 of 6) | ||
| PML-RARαm4 leukemia | 87 ± 7 | 92 ± 1 | ‡0.3, 32, 93 | NA | NA |
| no. 4048.2, 25 000 cells | (5 of 6) | (2 of 4) | (3 of 4) | ||
| PML-RARα leukemia | 80 ± 17 | NA | NA | 53 ± 25 | 17 ± 17 |
| no. 935, 25 000 cells | (4 of 4) | (6 of 6) | (6 of 6) |
| . | Control . | hC/EBPα . | hC/EBPɛ* . | rC/EBPα-WT† . | rC/EBPα-ΔuORF† . |
|---|---|---|---|---|---|
| PML-RARα leukemia | 67 ± 16 | 60 ± 8 | 4 ± 2 | NA | NA |
| no. 1111, 50 000 cells | (2 of 5) | (4 of 5) | (2 of 4) | ||
| PML-RARα leukemia | 88 ± 4 | 72 ± 9 | ‡0.2, 70, 80 | NA | NA |
| no. 1111, 25 000 cells | (3 of 4) | (4 of 5) | (3 of 5) | ||
| PML-RARα leukemia | 56 ± 12 | 59 ± 5 | 0.6 ± 0.3 | NA | NA |
| no. 935, 50 000 cells | (5 of 6) | (5 of 6) | (3 of 6) | ||
| PML-RARαm4 leukemia | 87 ± 7 | 92 ± 1 | ‡0.3, 32, 93 | NA | NA |
| no. 4048.2, 25 000 cells | (5 of 6) | (2 of 4) | (3 of 4) | ||
| PML-RARα leukemia | 80 ± 17 | NA | NA | 53 ± 25 | 17 ± 17 |
| no. 935, 25 000 cells | (4 of 4) | (6 of 6) | (6 of 6) |
Recipients of transduced leukemic cells were killed when visibly ill. Percentages of cells expressing GFP are shown. For most groups, values are means ± SDs. Values in parentheses indicate the number of mice in which GFP expression was assessed compared with total mice in the group.
NA indicates not applicable.
Some leukemias in hC/EBPɛ recipients do not express GFP.
The C/EBPα uORF tolerizes leukemic cells to the presence of C/EBPα-IRES-GFP retroviral transcript (rC/EBPα-WT versus rC/EBPα-ΔuORF; P = .01).
Values for individual mice are shown because of marked variations among mice.