Table 2. Global and Syk-specific... | American Society of Hematology

Table 2.

Global and Syk-specific tyrosine phosphorylation

Patient no.	V_H gene status	CD38 status	Phosphorylation with anti-IgM^*	Fold rise in Syk phosphorylation^†
1	Unmutated	+	Yes	3
2	Unmutated	+	Yes	4
3	Unmutated	+	Yes	3
4	Unmutated	+	Yes	3
5	Unmutated	+	Yes	3
6	Unmutated	+	No	0
7	Unmutated	+	Yes	3
8	Unmutated	+	No	0
9	Unmutated	+	Yes	3
10	Unmutated	+	No	0
11	Unmutated	−	Yes	2.5
12	Unmutated	−	Yes	2
13	Unmutated	−	Yes	2
14	Unmutated	−	Yes	2
15	Unmutated	−	Yes	2
16	Mutated	+	No	0
17	Mutated	+	No	0
18	Mutated	+	Yes	5
19	Mutated	+	Yes	2
20	Mutated	+	Yes	4
21	Mutated	+	Yes	3.5
22	Mutated	+	No	0
23	Mutated	ND	Yes	4
24	Mutated	−	No	0
25	Mutated	−	No	1.5
26	Mutated	−	No	0
27	Mutated	−	No	0
28	Mutated	−	No	0
29	Mutated	−	No	0
30	Mutated	−	No	0
31	Mutated	−	No	0
32	Mutated	−	No	0
33	Mutated	−	No	0
34	Mutated	ND	ND	0
35	Mutated	−	ND	0
36	Mutated	−	ND	1.8
37	Mutated	−	ND	0
38	Mutated	−	ND	0
39	Mutated	ND	ND	0
40	Mutated	−	ND	1.3

Patient no.	V_H gene status	CD38 status	Phosphorylation with anti-IgM^*	Fold rise in Syk phosphorylation^†
1	Unmutated	+	Yes	3
2	Unmutated	+	Yes	4
3	Unmutated	+	Yes	3
4	Unmutated	+	Yes	3
5	Unmutated	+	Yes	3
6	Unmutated	+	No	0
7	Unmutated	+	Yes	3
8	Unmutated	+	No	0
9	Unmutated	+	Yes	3
10	Unmutated	+	No	0
11	Unmutated	−	Yes	2.5
12	Unmutated	−	Yes	2
13	Unmutated	−	Yes	2
14	Unmutated	−	Yes	2
15	Unmutated	−	Yes	2
16	Mutated	+	No	0
17	Mutated	+	No	0
18	Mutated	+	Yes	5
19	Mutated	+	Yes	2
20	Mutated	+	Yes	4
21	Mutated	+	Yes	3.5
22	Mutated	+	No	0
23	Mutated	ND	Yes	4
24	Mutated	−	No	0
25	Mutated	−	No	1.5
26	Mutated	−	No	0
27	Mutated	−	No	0
28	Mutated	−	No	0
29	Mutated	−	No	0
30	Mutated	−	No	0
31	Mutated	−	No	0
32	Mutated	−	No	0
33	Mutated	−	No	0
34	Mutated	ND	ND	0
35	Mutated	−	ND	0
36	Mutated	−	ND	1.8
37	Mutated	−	ND	0
38	Mutated	−	ND	0
39	Mutated	ND	ND	0
40	Mutated	−	ND	1.3

The V_H gene and CD38 expression status are shown. Samples with CD38 expression on more than 30% of CD5/CD19 double-positive cells were considered positive.

ND indicates not determined.

CLL samples with a visible increase in global tyrosine phosphorylation in anti-IgM–stimulated cells relative to control antibody–treated cells were considered to be responsive to anti-IgM.

†

Fold increase in tyrosine phosphorylated Syk in anti-IgM–treated cells relative to control antibody–treated cells.

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