Clinicopathologic details of patients
| Patient no. . | Age, y . | Sex . | Stage . | WBC count, × 109/L . | VH gene . | VHgene status . | CD38*, % . | sIgM MEF† . | CD20 MEF† . | CD79β MEF† . |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 83 | M | A | 32.2 | 3-30 | Unmutated | 99 | 3700 | 7000 | 680 |
| 2 | 70 | F | B | 8.9 | 1-03 | Unmutated | 90 | + | + | + |
| 3 | 82 | M | A | 37.8 | 3-07 | Unmutated | 88 | 20 000 | 14 000 | 1400 |
| 4 | 93 | M | B | 219 | 4-59 | Unmutated | 79 | 16 000 | 9 500 | 850 |
| 5 | 52 | M | B | 150 | 3-09 | Unmutated | 76 | 23 000 | 25 000 | 800 |
| 6 | 55 | M | B | 30 | 3-30 | Unmutated | 72 | 1 300 | 8 100 | 700 |
| 7 | 80 | F | A | 143 | 2-70 | Unmutated | 41 | 15 000 | 7 200 | 4000 |
| 8 | 70 | M | A | 210 | 4-04 | Unmutated | 39 | 7 500 | 12 000 | 1600 |
| 9 | 74 | M | A | 33.3 | 1-69 | Unmutated | 35 | 17 000 | 13 000 | 820 |
| 10 | 86 | F | A | 50 | 3-30 | Unmutated | 33 | 23 000 | 11 000 | 2100 |
| 11 | 80 | M | A | 18.7 | 3-21 | Unmutated | 12 | 11 000 | 6 400 | 1300 |
| 12 | 70 | F | A | 106.8 | 3-33 | Unmutated | 7 | 4 400 | + | + |
| 13 | 49 | M | A | 117.2 | 4-59 | Unmutated | 6 | 5 400 | 9 200 | 450 |
| 14 | 87 | F | A | 12 | 3-11 | Unmutated | 5 | + | + | 460 |
| 15 | 69 | M | C | 30.1 | 3-73 | Unmutated | 3 | 43 000 | 14 000 | 2900 |
| 16 | 63 | M | A | 110 | 4-39 | Mutated | 99 | + | + | + |
| 17 | 79 | F | A | 15.7 | 3-15 | Mutated | 98 | + | + | 1700 |
| 18 | 85 | F | A | 181.7 | 3-23 | Mutated | 77 | 43 000 | 51 000 | 2400 |
| 19 | 69 | M | A | 70 | 1-69 | Mutated | 64 | 12 000 | 9 400 | 900 |
| 20 | 73 | M | A | 33 | 3-23 | Mutated | 55 | 2 200 | 6 800 | + |
| 21 | 88 | F | A | 150 | 3-72 | Mutated | 45 | 8 000 | 17 000 | 4400 |
| 22 | 86 | F | A | 24.7 | 3-23 | Mutated | 35 | 6 800 | 10 000 | 850 |
| 23 | 72 | F | A | 22.3 | 3-21 | Mutated | ND | + | + | + |
| 24 | 76 | F | A | 57.7 | 4-34 | Mutated | 18 | 16 000 | 20 000 | 680 |
| 25 | 78 | M | A | 40.5 | 4-34 | Mutated | 9 | 5 400 | 4 700 | 600 |
| 26 | 63 | M | A | 34 | 3-72 | Mutated | 6 | 8 000 | 7 900 | 700 |
| 27 | 60 | M | A | 142.7 | 3-48 | Mutated | 4 | 7 900 | 9 400 | 780 |
| 28 | 83 | F | A | 73.9 | 4-59 | Mutated | 1 | 23 000 | 7 200 | 750 |
| 29 | 76 | F | A | 61.2 | 2-26 | Mutated | 1 | + | + | + |
| 30 | 82 | M | A | 42.2 | 3-30 | Mutated | 1 | + | + | + |
| 31 | 38 | M | A | 35.9 | 4-34 | Mutated | 1 | + | + | + |
| 32 | 73 | F | A | 60 | 3-23 | Mutated | 1 | 6 500 | 6 400 | 1200 |
| 33 | 45 | M | A | 121.4 | 3-21 | Mutated | 10 | + | + | + |
| 34 | 57 | F | A | 22.1 | 2-05 | Mutated | ND | + | + | + |
| 35 | 88 | M | A | 17.9 | 3-30 | Mutated | 0 | + | + | + |
| 36 | 86 | F | A | 8.4 | 1-69 | Mutated | 11 | + | + | + |
| 37 | 57 | F | A | 10.2 | 3-53 | Mutated | 1 | + | + | + |
| 38 | 57 | F | A | 84 | 4-34 | Mutated | 7 | + | + | + |
| 39 | 79 | F | A | 15.7 | 6-01 | Mutated | ND | + | + | + |
| 40 | 70 | M | A | 17.9 | 4-59 | Mutated | 8 | + | + | + |
| Patient no. . | Age, y . | Sex . | Stage . | WBC count, × 109/L . | VH gene . | VHgene status . | CD38*, % . | sIgM MEF† . | CD20 MEF† . | CD79β MEF† . |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 83 | M | A | 32.2 | 3-30 | Unmutated | 99 | 3700 | 7000 | 680 |
| 2 | 70 | F | B | 8.9 | 1-03 | Unmutated | 90 | + | + | + |
| 3 | 82 | M | A | 37.8 | 3-07 | Unmutated | 88 | 20 000 | 14 000 | 1400 |
| 4 | 93 | M | B | 219 | 4-59 | Unmutated | 79 | 16 000 | 9 500 | 850 |
| 5 | 52 | M | B | 150 | 3-09 | Unmutated | 76 | 23 000 | 25 000 | 800 |
| 6 | 55 | M | B | 30 | 3-30 | Unmutated | 72 | 1 300 | 8 100 | 700 |
| 7 | 80 | F | A | 143 | 2-70 | Unmutated | 41 | 15 000 | 7 200 | 4000 |
| 8 | 70 | M | A | 210 | 4-04 | Unmutated | 39 | 7 500 | 12 000 | 1600 |
| 9 | 74 | M | A | 33.3 | 1-69 | Unmutated | 35 | 17 000 | 13 000 | 820 |
| 10 | 86 | F | A | 50 | 3-30 | Unmutated | 33 | 23 000 | 11 000 | 2100 |
| 11 | 80 | M | A | 18.7 | 3-21 | Unmutated | 12 | 11 000 | 6 400 | 1300 |
| 12 | 70 | F | A | 106.8 | 3-33 | Unmutated | 7 | 4 400 | + | + |
| 13 | 49 | M | A | 117.2 | 4-59 | Unmutated | 6 | 5 400 | 9 200 | 450 |
| 14 | 87 | F | A | 12 | 3-11 | Unmutated | 5 | + | + | 460 |
| 15 | 69 | M | C | 30.1 | 3-73 | Unmutated | 3 | 43 000 | 14 000 | 2900 |
| 16 | 63 | M | A | 110 | 4-39 | Mutated | 99 | + | + | + |
| 17 | 79 | F | A | 15.7 | 3-15 | Mutated | 98 | + | + | 1700 |
| 18 | 85 | F | A | 181.7 | 3-23 | Mutated | 77 | 43 000 | 51 000 | 2400 |
| 19 | 69 | M | A | 70 | 1-69 | Mutated | 64 | 12 000 | 9 400 | 900 |
| 20 | 73 | M | A | 33 | 3-23 | Mutated | 55 | 2 200 | 6 800 | + |
| 21 | 88 | F | A | 150 | 3-72 | Mutated | 45 | 8 000 | 17 000 | 4400 |
| 22 | 86 | F | A | 24.7 | 3-23 | Mutated | 35 | 6 800 | 10 000 | 850 |
| 23 | 72 | F | A | 22.3 | 3-21 | Mutated | ND | + | + | + |
| 24 | 76 | F | A | 57.7 | 4-34 | Mutated | 18 | 16 000 | 20 000 | 680 |
| 25 | 78 | M | A | 40.5 | 4-34 | Mutated | 9 | 5 400 | 4 700 | 600 |
| 26 | 63 | M | A | 34 | 3-72 | Mutated | 6 | 8 000 | 7 900 | 700 |
| 27 | 60 | M | A | 142.7 | 3-48 | Mutated | 4 | 7 900 | 9 400 | 780 |
| 28 | 83 | F | A | 73.9 | 4-59 | Mutated | 1 | 23 000 | 7 200 | 750 |
| 29 | 76 | F | A | 61.2 | 2-26 | Mutated | 1 | + | + | + |
| 30 | 82 | M | A | 42.2 | 3-30 | Mutated | 1 | + | + | + |
| 31 | 38 | M | A | 35.9 | 4-34 | Mutated | 1 | + | + | + |
| 32 | 73 | F | A | 60 | 3-23 | Mutated | 1 | 6 500 | 6 400 | 1200 |
| 33 | 45 | M | A | 121.4 | 3-21 | Mutated | 10 | + | + | + |
| 34 | 57 | F | A | 22.1 | 2-05 | Mutated | ND | + | + | + |
| 35 | 88 | M | A | 17.9 | 3-30 | Mutated | 0 | + | + | + |
| 36 | 86 | F | A | 8.4 | 1-69 | Mutated | 11 | + | + | + |
| 37 | 57 | F | A | 10.2 | 3-53 | Mutated | 1 | + | + | + |
| 38 | 57 | F | A | 84 | 4-34 | Mutated | 7 | + | + | + |
| 39 | 79 | F | A | 15.7 | 6-01 | Mutated | ND | + | + | + |
| 40 | 70 | M | A | 17.9 | 4-59 | Mutated | 8 | + | + | + |
The VH gene expressed and its mutation status are shown for each patient. A greater than 2% deviation from germline VH sequence was considered as mutated. All patients expressed surface IgM and IgD. Disease stage is according to Binet scale. WBC indicates white blood cell; +, positive expression by FACS analysis, however expression level was not determined using MEF; ND, not determined.
The percentage of CD19+ and CD5+ cells coexpressing CD38 was determined by flow cytometry.
sIgM, CD79β, and CD20 expression levels are expressed as mean equivalent fluorochrome values.