Table 3.

Flow cytometric analysis of the band 3 Coimbra RBCs

Monoclonal antibodyAntigenControl, FL1 unitsBand 3 Coimbra, FL1 units
BRIC169 Mouse negative control 2.36 ± 0.08 2.49  
BRIC6 Band 3 334 ± 23 2.67  
BRIC71 Band 3 1035 ± 525 26.2 
BRIC90 Band 3 672 ± 89 3.02  
BRIC200 Band 3 1379 ± 330 2.42  
B6 H12.2 CD47 1081 ± 100 159 
R10 GPA 1595 ± 107 3240 
BRIC256 GPA 840 ± 59 614 
BRIC14 Wrb 1991 ± 631 1670 
BRIC201 Wrb 92.5 ± 13.6 2.37 
BRIC4 GPC 888 ± 59 846  
BS 46 LW 30.9 ± 8.9 9.54 
BRIC110 Daf 29.3 ± 3.6 38.0 
BRIC68 Kell 140 ± 10 152  
BRIC69 Rh polypeptides 548 ± 49 181 
LA1818 RhAG 1820 ± 72 267 
Monoclonal antibodyAntigenControl, FL1 unitsBand 3 Coimbra, FL1 units
BRIC169 Mouse negative control 2.36 ± 0.08 2.49  
BRIC6 Band 3 334 ± 23 2.67  
BRIC71 Band 3 1035 ± 525 26.2 
BRIC90 Band 3 672 ± 89 3.02  
BRIC200 Band 3 1379 ± 330 2.42  
B6 H12.2 CD47 1081 ± 100 159 
R10 GPA 1595 ± 107 3240 
BRIC256 GPA 840 ± 59 614 
BRIC14 Wrb 1991 ± 631 1670 
BRIC201 Wrb 92.5 ± 13.6 2.37 
BRIC4 GPC 888 ± 59 846  
BS 46 LW 30.9 ± 8.9 9.54 
BRIC110 Daf 29.3 ± 3.6 38.0 
BRIC68 Kell 140 ± 10 152  
BRIC69 Rh polypeptides 548 ± 49 181 
LA1818 RhAG 1820 ± 72 267 

FL1 units are mean fluorescence intensity obtained on flow cytometry. Control values are the mean (± SD) of 3 control RBC samples.

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