Quantification of the membrane proteins of band 3 Coimbra RBCs by immunoblotting and Coomassie blue staining
| Protein . | Antibody . | Band 3 Coimbra, % of controls* . | n . |
|---|---|---|---|
| Band 3 | N-terminal BRIC170 | 2, 4 | 2 |
| Band 3 | C-terminal BRIC155 | 1, 2 | 2 |
| Protein 4.2 | Polyclonal | 0 | 2 |
| GPA, total | BRIC163 | 29 (± 5) | 6 |
| RhAG | LA1818 | 7 (± 1) | 4 |
| Rh polypeptides | Polyclonal | 21 (± 3) | 6 |
| CD47 | BRIC125 | 3 (± 1) | 6 |
| CD47 | C-terminal polyclonal | 2 (± 1) | 6 |
| GPB, total | R1.3 | 56 (± 3) | 6 |
| LW | BS56 | 5, 9 | 2 |
| Glycophorin C | BRIC4 | 100 (± 7) | 8 |
| Protein 4.1 | Coomassie | 94 (± 6) | 4 |
| Protein 4.1 | Polyclonal | 101 (± 7) | 4 |
| p55 | Polyclonal | 108, 115 | 2 |
| Spectrin | Coomassie | 64 (± 1) | 4 |
| α-spectrin | Monoclonal | 65, 61 | 2 |
| β-spectrin | Monoclonal | 58, 67 | 2 |
| β-actin | Monoclonal | 71 (± 3) | 4 |
| AQP1, total | Polyclonal | 59 (± 3) | 4 |
| GLUT 1, total | Polyclonal | 182 (± 10) | 4 |
| Lu | BRIC221 | 169 (± 35) | 4 |
| LFA-3, CD58 | BRIC5 | 315, 325 | 2 |
| DAF, CD55 | BRIC128 | 163 (± 15) | 4 |
| CD44 | BRIC235 | 113, 118 | 2 |
| Protein . | Antibody . | Band 3 Coimbra, % of controls* . | n . |
|---|---|---|---|
| Band 3 | N-terminal BRIC170 | 2, 4 | 2 |
| Band 3 | C-terminal BRIC155 | 1, 2 | 2 |
| Protein 4.2 | Polyclonal | 0 | 2 |
| GPA, total | BRIC163 | 29 (± 5) | 6 |
| RhAG | LA1818 | 7 (± 1) | 4 |
| Rh polypeptides | Polyclonal | 21 (± 3) | 6 |
| CD47 | BRIC125 | 3 (± 1) | 6 |
| CD47 | C-terminal polyclonal | 2 (± 1) | 6 |
| GPB, total | R1.3 | 56 (± 3) | 6 |
| LW | BS56 | 5, 9 | 2 |
| Glycophorin C | BRIC4 | 100 (± 7) | 8 |
| Protein 4.1 | Coomassie | 94 (± 6) | 4 |
| Protein 4.1 | Polyclonal | 101 (± 7) | 4 |
| p55 | Polyclonal | 108, 115 | 2 |
| Spectrin | Coomassie | 64 (± 1) | 4 |
| α-spectrin | Monoclonal | 65, 61 | 2 |
| β-spectrin | Monoclonal | 58, 67 | 2 |
| β-actin | Monoclonal | 71 (± 3) | 4 |
| AQP1, total | Polyclonal | 59 (± 3) | 4 |
| GLUT 1, total | Polyclonal | 182 (± 10) | 4 |
| Lu | BRIC221 | 169 (± 35) | 4 |
| LFA-3, CD58 | BRIC5 | 315, 325 | 2 |
| DAF, CD55 | BRIC128 | 163 (± 15) | 4 |
| CD44 | BRIC235 | 113, 118 | 2 |
Values given as the mean (± SD) of n replicates. Amount of protein expressed as the percentage of the control after normalization to the amount of GPC in the band 3 Coimbra membrane preparation.