Table 1.

Antibodies used in the analyses, indicating source, dilution, threshold, and pattern of reactivity used, and positive controls

ProteinCloneSourceDilutionReactivityThresholdInternal control
Ki67 M1B1 DAKO 1:100 High/low >50% positive H/RS cells Proliferating cells 
Bcl2 124 DAKO 1:25 High/low >50% positive H/RS cells with strong expression Small lymphocytes 
Bax POLYCLONAL Santa Cruz 1:1000 Positive/negative >10% positive H/RS cells Benign B lymphocytes 
Bcl-XL 2H12 ZYMED 1:10 High/low >50% positive H/RS cells with strong expression HL-derived cell lines 
Mcl1 POLYCLONAL DAKO 1:100 High/low >50% positive H/RS cells with strong expression Proliferating cells 
Survivin POLYCLONAL RD Systems 1:1500 High/low >50% positive H/RS cells with strong expression HL-derived cell lines  
p65/RelA F-6 (p65) Santa Cruz 1:2000 Positive/negative Nuclear expression in H/RS cells HL-derived cell lines  
Cyclin A 6E6 Novocastra 1:100 Positive/negative >10% positive H/RS cells Proliferating cells (G2/M)  
Cyclin B1 7A9 Novocastra 1:25 Positive/negative >10% positive H/RS cells Proliferating cells (G2/M)  
Cyclin D1 DCS-6 DAKO 1:100 Positive/negative Any positive H/RS cells Macrophages and endothelial cells  
Cyclin D3 DCS-22 Novocastra 1:10 Positive/negative >10% positive H/RS cells Proliferating cells  
Cyclin E 13A3 Novocastra 1:10 Positive/negative >10% positive H/RS cells TMA controls, proliferating cells 
CDK1 1 Transduction Lab 1:1500 Positive/negative >10% positive H/RS cells TMA controls, proliferating cells 
CDK2 8D4 NeoMarkers 1:500 Positive/negative >10% positive H/RS cells TMA controls, proliferating cells 
CDK6 K6.83 Chemicon 1:10 Positive/negative >10% positive H/RS cells TMA controls 
Bcl6 PG-B6p DAKO 1:10 Positive/negative >10% positive H/RS cells CG B cells and B-cell lymphomas 
SKP2 1G12E9 ZYMED 1:10 Positive/negative >50% positive H/RS cells Proliferating cells  
STAT1 C-136 Santa Cruz 1:50 Positive/negative Nuclear expression in H/RS cells Reactive lymphocytes and macrophages  
STAT3 F-2 Santa Cruz 1:500 Positive/negative Nuclear expression in H/RS cells Reactive lymphocytes and macrophages 
PTEN 28H6 Novocastra 1:500 Positive/negative Expression in H/RS cells similar to benign cells Normal cells 
P53 DO-7 Novocastra 1:50 High/low >80% positive H/RS cells Scattered GC cells 
P21 EA10 Oncogene 1:50 High/low >50% positive H/RS cells Scattered GC cells  
P16 POLICLONAL Santa Cruz 1:50 High/low Expression in H/RS cells similar to benign cells Normal cells  
P27 57 Transduction Lab 1:1000 High/low >50% positive H/RS cells Resting lymphoid cells  
Hdm2 IF2 (Mdm2) Oncogen 1:10 High/low >50% positive H/RS cells TMA internal controls, macrophages,
and endothelial cells 
Rb G3-245 BD PharMingen 1:250 High/low >50% positive H/RS cells Proliferating cells 
EBV-LMP CS1-4 Novocastra 1:250 Positive/negative Cytoplasmic and membranous expression in H/RS EBV-positive TMA controls 
ProteinCloneSourceDilutionReactivityThresholdInternal control
Ki67 M1B1 DAKO 1:100 High/low >50% positive H/RS cells Proliferating cells 
Bcl2 124 DAKO 1:25 High/low >50% positive H/RS cells with strong expression Small lymphocytes 
Bax POLYCLONAL Santa Cruz 1:1000 Positive/negative >10% positive H/RS cells Benign B lymphocytes 
Bcl-XL 2H12 ZYMED 1:10 High/low >50% positive H/RS cells with strong expression HL-derived cell lines 
Mcl1 POLYCLONAL DAKO 1:100 High/low >50% positive H/RS cells with strong expression Proliferating cells 
Survivin POLYCLONAL RD Systems 1:1500 High/low >50% positive H/RS cells with strong expression HL-derived cell lines  
p65/RelA F-6 (p65) Santa Cruz 1:2000 Positive/negative Nuclear expression in H/RS cells HL-derived cell lines  
Cyclin A 6E6 Novocastra 1:100 Positive/negative >10% positive H/RS cells Proliferating cells (G2/M)  
Cyclin B1 7A9 Novocastra 1:25 Positive/negative >10% positive H/RS cells Proliferating cells (G2/M)  
Cyclin D1 DCS-6 DAKO 1:100 Positive/negative Any positive H/RS cells Macrophages and endothelial cells  
Cyclin D3 DCS-22 Novocastra 1:10 Positive/negative >10% positive H/RS cells Proliferating cells  
Cyclin E 13A3 Novocastra 1:10 Positive/negative >10% positive H/RS cells TMA controls, proliferating cells 
CDK1 1 Transduction Lab 1:1500 Positive/negative >10% positive H/RS cells TMA controls, proliferating cells 
CDK2 8D4 NeoMarkers 1:500 Positive/negative >10% positive H/RS cells TMA controls, proliferating cells 
CDK6 K6.83 Chemicon 1:10 Positive/negative >10% positive H/RS cells TMA controls 
Bcl6 PG-B6p DAKO 1:10 Positive/negative >10% positive H/RS cells CG B cells and B-cell lymphomas 
SKP2 1G12E9 ZYMED 1:10 Positive/negative >50% positive H/RS cells Proliferating cells  
STAT1 C-136 Santa Cruz 1:50 Positive/negative Nuclear expression in H/RS cells Reactive lymphocytes and macrophages  
STAT3 F-2 Santa Cruz 1:500 Positive/negative Nuclear expression in H/RS cells Reactive lymphocytes and macrophages 
PTEN 28H6 Novocastra 1:500 Positive/negative Expression in H/RS cells similar to benign cells Normal cells 
P53 DO-7 Novocastra 1:50 High/low >80% positive H/RS cells Scattered GC cells 
P21 EA10 Oncogene 1:50 High/low >50% positive H/RS cells Scattered GC cells  
P16 POLICLONAL Santa Cruz 1:50 High/low Expression in H/RS cells similar to benign cells Normal cells  
P27 57 Transduction Lab 1:1000 High/low >50% positive H/RS cells Resting lymphoid cells  
Hdm2 IF2 (Mdm2) Oncogen 1:10 High/low >50% positive H/RS cells TMA internal controls, macrophages,
and endothelial cells 
Rb G3-245 BD PharMingen 1:250 High/low >50% positive H/RS cells Proliferating cells 
EBV-LMP CS1-4 Novocastra 1:250 Positive/negative Cytoplasmic and membranous expression in H/RS EBV-positive TMA controls 
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