Primers and conditions used for bisulfite PCR and RT-PCR
| Primers . | Sequences . | GenBank accession no. . | Temperature, °C (cycles) . | Size, bp . | Enzyme . |
|---|---|---|---|---|---|
| P57KIP2 | F: 5′-GGTTGGGYGTTTTATAGGTTA-3′ | D64137 | 58 (3), 56 (4), 54 (5), 52 (26) | 155 | EcoRI |
| R: 5′-ACCTAACTATCCGATAATAAACTCTTC-3′ | HhaI | ||||
| P21CIP1 | F: 5′-GGYGGGGYGGTTGTATATTAG-3′ | U24170 | 60 (3), 57 (4), 54 (5), 51 (23) | 168 | BstUI |
| R: 5′-CRACCCRAAATCCCCTATTATC-3′ | |||||
| P14ARF | F: 5′-TTTYGGGGYGGAGATGGGT-3′ | AF082338 | 58 (3), 56 (4), 54 (5), 52 (23) | 160 | TaqI |
| R: 5′-ATCACCAAAAACCTACRCACCATATTC-3′ | |||||
| p57RT | F: 5′-CTGACCAGCTGCACTCGGGGATTTC-3′ | — | 60 (35) | 341 | — |
| R: 5′-GCCGCCGGTTGCTGCTACATGA-3′ |
| Primers . | Sequences . | GenBank accession no. . | Temperature, °C (cycles) . | Size, bp . | Enzyme . |
|---|---|---|---|---|---|
| P57KIP2 | F: 5′-GGTTGGGYGTTTTATAGGTTA-3′ | D64137 | 58 (3), 56 (4), 54 (5), 52 (26) | 155 | EcoRI |
| R: 5′-ACCTAACTATCCGATAATAAACTCTTC-3′ | HhaI | ||||
| P21CIP1 | F: 5′-GGYGGGGYGGTTGTATATTAG-3′ | U24170 | 60 (3), 57 (4), 54 (5), 51 (23) | 168 | BstUI |
| R: 5′-CRACCCRAAATCCCCTATTATC-3′ | |||||
| P14ARF | F: 5′-TTTYGGGGYGGAGATGGGT-3′ | AF082338 | 58 (3), 56 (4), 54 (5), 52 (23) | 160 | TaqI |
| R: 5′-ATCACCAAAAACCTACRCACCATATTC-3′ | |||||
| p57RT | F: 5′-CTGACCAGCTGCACTCGGGGATTTC-3′ | — | 60 (35) | 341 | — |
| R: 5′-GCCGCCGGTTGCTGCTACATGA-3′ |
F indicates forward; R, reverse; —, not applicable.