CMV UL97 and UL54 amino acid substitutions in HSCT recipients with late-onset CMV disease
| Specimen . | Days after HSCT . | Cumulative gan/fos, d . | Specimen type . | UL97 amino acid substitution . | UL54amino acid substitution . |
|---|---|---|---|---|---|
| Patient 1 | |||||
| A | 54 | 21/14 | Blood | WT | — |
| B | 131 | 98/14 | Blood | WT | — |
| C | 264 | 231/40 | Blood | Leu595Phe*; WT | — |
| D | 278 | 245/40 | Blood | Leu595Phe*; WT | — |
| E | 285 | 252/40 | Blood | Leu595Phe* | WT Pro628Leu, Ser655Leu, Asn685Ser, Ala885Ser, 885T insertion |
| F | CSF | Cys603Trp*; WT | WT strain 1: Pro628Leu, Ser655Leu, Asn685Ser, Ala885Ser, 885T insertion (same sequence as E above). Strain 2 (partial sequence): 16 nucleotide differences, no amino acid differences from strain 1 | ||
| Patient 2 | |||||
| A | 52 | 3/0 | Blood | WT | — |
| B | 145 | 82/0 | Blood | WT | — |
| C | 201 | 103/28 | Blood | WT | WT Ser655Leu, Asn685Ser, Ala885Thr, Asn898Asp |
| D | 204 | 106/31 | BAL | WT | WT Ser655Leu, Asn685Ser, Ala885Thr, Asn898Asp |
| E | 205 | 107/32 | CSF | WT | WT Ser655Leu, Asn685Ser, Ala885Thr, Asn898Asp |
| F | 212 | 114/39 | Lung biopsy | Cys592Gly*; WT | WT Ser655Ley, Asn685Ser, Ala885Thr, Asn898Asp |
| Specimen . | Days after HSCT . | Cumulative gan/fos, d . | Specimen type . | UL97 amino acid substitution . | UL54amino acid substitution . |
|---|---|---|---|---|---|
| Patient 1 | |||||
| A | 54 | 21/14 | Blood | WT | — |
| B | 131 | 98/14 | Blood | WT | — |
| C | 264 | 231/40 | Blood | Leu595Phe*; WT | — |
| D | 278 | 245/40 | Blood | Leu595Phe*; WT | — |
| E | 285 | 252/40 | Blood | Leu595Phe* | WT Pro628Leu, Ser655Leu, Asn685Ser, Ala885Ser, 885T insertion |
| F | CSF | Cys603Trp*; WT | WT strain 1: Pro628Leu, Ser655Leu, Asn685Ser, Ala885Ser, 885T insertion (same sequence as E above). Strain 2 (partial sequence): 16 nucleotide differences, no amino acid differences from strain 1 | ||
| Patient 2 | |||||
| A | 52 | 3/0 | Blood | WT | — |
| B | 145 | 82/0 | Blood | WT | — |
| C | 201 | 103/28 | Blood | WT | WT Ser655Leu, Asn685Ser, Ala885Thr, Asn898Asp |
| D | 204 | 106/31 | BAL | WT | WT Ser655Leu, Asn685Ser, Ala885Thr, Asn898Asp |
| E | 205 | 107/32 | CSF | WT | WT Ser655Leu, Asn685Ser, Ala885Thr, Asn898Asp |
| F | 212 | 114/39 | Lung biopsy | Cys592Gly*; WT | WT Ser655Ley, Asn685Ser, Ala885Thr, Asn898Asp |
gan indicates ganciclovir; fos, foscarnet; WT, wild-type sequences; CSF, cerebrospinal fluid; BAL, bronchoalveolar lavage; and —, not available.
Amino acid substitutions known to be associated with resistance.