Engraftment of hCD45+ cells in bone marrow and peripheral blood in NOD/SCID mice
. | Mice with reconstituted cells/transplant recipients, no. . | CD45+ cells in BM of recipients, % . | CD45+ cells in PB of recipients, % . |
---|---|---|---|
Accessory cells | 0/3 | ND | ND |
Precoculture | 4/5 | 1.67 ± 2.06 | ND |
Stroma free, 2 wk | 0/3 | ND | ND |
Primary, 2 wk | 3/5 | 0.79 ± 1.00 | ND |
hTERT, 2 wk | 5/5 | 1.65 ± 1.33 | ND |
Primary, 4 wk | 5/5 | 5.88 ± 4.86 | 0.15 ± 0.09 |
hTERT, 4 wk | 5/5 | 9.67 ± 5.57* | 0.27 ± 0.23 |
. | Mice with reconstituted cells/transplant recipients, no. . | CD45+ cells in BM of recipients, % . | CD45+ cells in PB of recipients, % . |
---|---|---|---|
Accessory cells | 0/3 | ND | ND |
Precoculture | 4/5 | 1.67 ± 2.06 | ND |
Stroma free, 2 wk | 0/3 | ND | ND |
Primary, 2 wk | 3/5 | 0.79 ± 1.00 | ND |
hTERT, 2 wk | 5/5 | 1.65 ± 1.33 | ND |
Primary, 4 wk | 5/5 | 5.88 ± 4.86 | 0.15 ± 0.09 |
hTERT, 4 wk | 5/5 | 9.67 ± 5.57* | 0.27 ± 0.23 |
ND indicates hCD45% values below the cutoff level (0.1%) ± standard deviation.
P < .05, compared with precoculture group (Mann-Whitney U test).