Distribution of gene expression in purified hematopoietic stem and progenitor cells
| . | HSCs . | MPPs . | CLPs . | CMPs . |
|---|---|---|---|---|
| Presence of expression | ||||
| Genes with expression levels passed cutoff line (more than 100) | 10 677 (43%) | 10 488 (42%) | 10 466 (42%) | 10 455 (42%) |
| ESTs (Affymetrix database) | 8 600 | 8 402 | 8 438 | 8 471 |
| Known genes | 2 077 | 2 086 | 2 028 | 1 984 |
| Genes affiliated to nonhematopoietic tissues | 58 | 58 | 58 | 58 |
| Genes with normalized value more than 0.3 | 43 (74%) | 22 (37%) | 13 (22%) | 4 (7%) |
| Genes affiliated to hematopoiesis | 312 | 312 | 312 | 312 |
| Genes with normalized value more than 0.3 | 133 (42%) | 95 (30%) | 141 (45%) | 80(26%) |
| Low-level expression (more than 100 but less than 300) | 5 891 (24%) | 5 840 (23%) | 5 826 (23%) | 5 844 (23%) |
| Differential expression | ||||
| Genes passed screening filter (Figure 2) | 5 223 | 5 223 | 5 223 | 5 223 |
| Genes dominantly expressed in each population | 824 (16%) | 723 (13.8%) | 527 (11%) | 665 (12.7%) |
| ESTs (Affymetrix database) | 666 | 609 | 398 | 545 |
| Known genes | 158 | 114 | 129 | 120 |
| Genes affiliated to nonhematopoietic tissues | 48 | 48 | 48 | 48 |
| Genes with normalized value more than 0.3 | 35 (73%) | 18 (37%) | 10 (20%) | 4 (8.3%) |
| Genes affiliated to hematopoiesis | 162 | 162 | 162 | 162 |
| Genes with normalized value more than 0.3 | 63 (39%) | 42 (26%) | 67 (41%) | 40 (25%) |
| . | HSCs . | MPPs . | CLPs . | CMPs . |
|---|---|---|---|---|
| Presence of expression | ||||
| Genes with expression levels passed cutoff line (more than 100) | 10 677 (43%) | 10 488 (42%) | 10 466 (42%) | 10 455 (42%) |
| ESTs (Affymetrix database) | 8 600 | 8 402 | 8 438 | 8 471 |
| Known genes | 2 077 | 2 086 | 2 028 | 1 984 |
| Genes affiliated to nonhematopoietic tissues | 58 | 58 | 58 | 58 |
| Genes with normalized value more than 0.3 | 43 (74%) | 22 (37%) | 13 (22%) | 4 (7%) |
| Genes affiliated to hematopoiesis | 312 | 312 | 312 | 312 |
| Genes with normalized value more than 0.3 | 133 (42%) | 95 (30%) | 141 (45%) | 80(26%) |
| Low-level expression (more than 100 but less than 300) | 5 891 (24%) | 5 840 (23%) | 5 826 (23%) | 5 844 (23%) |
| Differential expression | ||||
| Genes passed screening filter (Figure 2) | 5 223 | 5 223 | 5 223 | 5 223 |
| Genes dominantly expressed in each population | 824 (16%) | 723 (13.8%) | 527 (11%) | 665 (12.7%) |
| ESTs (Affymetrix database) | 666 | 609 | 398 | 545 |
| Known genes | 158 | 114 | 129 | 120 |
| Genes affiliated to nonhematopoietic tissues | 48 | 48 | 48 | 48 |
| Genes with normalized value more than 0.3 | 35 (73%) | 18 (37%) | 10 (20%) | 4 (8.3%) |
| Genes affiliated to hematopoiesis | 162 | 162 | 162 | 162 |
| Genes with normalized value more than 0.3 | 63 (39%) | 42 (26%) | 67 (41%) | 40 (25%) |
The total of genes on the chips was identical at 24 818 for HSCs, MPPs, CLPs, and CMPs. Presence of expression was defined as follows: if the expression level of a given gene is more than 100. Genes that are highly expressed were defined as if a normalized value more than 0.3, here 0.3, is a normalized value (see the color bar for reference).