Table 2. Gene expression analysis by... | American Society of Hematology

Table 2.

Gene expression analysis by real-time quantitative PCR

		1 h			3 h			8 h
Probe	UNIGENE	C_TFn ± SD	C_T PLL ± SD	ΔC_T1h	C_T Fn ± SD	C_T PLL ± SD	ΔC_T 3h	C_T Fn ± SD	C_TPLL ± SD	ΔC_T 8h
1704_at	VAV2	33.20 ± 0.63	30.15 ± 0.26	+3.04	33.16 ± 0.78	31.07 ± 0.33	+2.09	34.59 ± 0.69	34.23 ± 0.71	+0.36
38976_at	CORO1A	22.28 ± 0.18	21.38 ± 0.09	+0.90	21.27 ± 0.05	22.41 ± 0.03	−1.13	20.28 ± 0.00	20.57 ± 0.16	−0.29
33917_at	EPB41L1	26.26 ± 0.41	26.12 ± 0.22	+0.14	24.04 ± 0.25	24.02 ± 0.48	+0.02	25.15 ± 0.37	24.18 ± 0.49	+0.97
32954_at	CCT4	21.62 ± 0.28	20.71 ± 0.32	+0.90	21.53 ± 0.18	22.50 ± 0.27	−0.97	20.89 ± 0.10	21.85 ± 0.37	−0.96
40139_at	FRAP1	25.55 ± 0.09	24.48 ± 0.56	+1.07	24.67 ± 0.09	25.41 ± 0.07	−0.74	24.24 ± 0.90	24.63 ± 0.18	−0.39
41076_at	GJB3	36.01 ± 0.20	40.85 ± 0.46	−4.84	32.09 ± 0.46	33.57 ± 0.78	−1.48	32.614 ± 0.48	32.87 ± 0.97	−0.26

		1 h			3 h			8 h
Probe	UNIGENE	C_TFn ± SD	C_T PLL ± SD	ΔC_T1h	C_T Fn ± SD	C_T PLL ± SD	ΔC_T 3h	C_T Fn ± SD	C_TPLL ± SD	ΔC_T 8h
1704_at	VAV2	33.20 ± 0.63	30.15 ± 0.26	+3.04	33.16 ± 0.78	31.07 ± 0.33	+2.09	34.59 ± 0.69	34.23 ± 0.71	+0.36
38976_at	CORO1A	22.28 ± 0.18	21.38 ± 0.09	+0.90	21.27 ± 0.05	22.41 ± 0.03	−1.13	20.28 ± 0.00	20.57 ± 0.16	−0.29
33917_at	EPB41L1	26.26 ± 0.41	26.12 ± 0.22	+0.14	24.04 ± 0.25	24.02 ± 0.48	+0.02	25.15 ± 0.37	24.18 ± 0.49	+0.97
32954_at	CCT4	21.62 ± 0.28	20.71 ± 0.32	+0.90	21.53 ± 0.18	22.50 ± 0.27	−0.97	20.89 ± 0.10	21.85 ± 0.37	−0.96
40139_at	FRAP1	25.55 ± 0.09	24.48 ± 0.56	+1.07	24.67 ± 0.09	25.41 ± 0.07	−0.74	24.24 ± 0.90	24.63 ± 0.18	−0.39
41076_at	GJB3	36.01 ± 0.20	40.85 ± 0.46	−4.84	32.09 ± 0.46	33.57 ± 0.78	−1.48	32.614 ± 0.48	32.87 ± 0.97	−0.26

The RNA expression level of 3 genes induced by PLL attachment (VAV2, CORO1A, EPB41L1) and 3 genes induced by Fn (CCT4, FRAP1, GJB3) was analyzed by real-time quantitative PCR experiments using SYBR Green. Mean values ± SDs are shown for Fn (C_T Fn) and PLL (C_T PLL), as well as the difference between the means (ΔC_T). A lower C_T value is indicative of a higher mRNA expression. The expression profile plots obtained for Nalm6 (except FRAP plot) corresponding to each gene analyzed by RT-Q-PCR are shown on the left side of the table.

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