Table 1. Confirmation of select... | American Society of Hematology

Table 1.

Confirmation of select microarray results by quantitative RT-PCR

Gene name	Accession no.	Microarray results, mean fold change: PC/B^*	RT-PCR C_T,^†PC	RT-PCR C_T, B	RT-PCR results, fold change: PC/B^‡
TBP	D01034	0.13	26.484	24.091	0.19
UBF	X60831	0.23	22.775	20.372	0.19
SOCS3	U88328	0.086	27.456	24.961	0.18
Axin	AF009011	2.53 × 10⁻³	24.311	22.037	0.21
TCF-1	X61385	4.86	24.598	23.212	0.38
TCF-1	AI019193	0.25	ND^1-155	ND	ND
Notch-1	Z11886	955.88	24.812	25.232	1.34
Pim-1	M13945	21.22	23.838	26.82	7.90
Reelin	U24703	321.59	24.840	≥ 40	≥ 3.66 × 104
Neuropilin-1	D50086	6.59	25.123	28.424	9.86
NFATcI	AF087434	0.16	25.275	22.789	0.18
NF-κB1	M57999	0.086	23.418	20.675	0.15
Stat6	L47650	0.064	24.546	21.152	0.095
CIITA	AF042158	0.048	38.072	21.096	7.8 × 10⁻⁶
Blimp-1	U08185	29.43	23.3	≥ 40	≥ 1.06 × 105
XBP-1	AW123880	17.15	17.492	21.738	18.97
PGK^1-153	M15668	1.13	21.397	21.588	1.14

Gene name	Accession no.	Microarray results, mean fold change: PC/B^*	RT-PCR C_T,^†PC	RT-PCR C_T, B	RT-PCR results, fold change: PC/B^‡
TBP	D01034	0.13	26.484	24.091	0.19
UBF	X60831	0.23	22.775	20.372	0.19
SOCS3	U88328	0.086	27.456	24.961	0.18
Axin	AF009011	2.53 × 10⁻³	24.311	22.037	0.21
TCF-1	X61385	4.86	24.598	23.212	0.38
TCF-1	AI019193	0.25	ND^1-155	ND	ND
Notch-1	Z11886	955.88	24.812	25.232	1.34
Pim-1	M13945	21.22	23.838	26.82	7.90
Reelin	U24703	321.59	24.840	≥ 40	≥ 3.66 × 104
Neuropilin-1	D50086	6.59	25.123	28.424	9.86
NFATcI	AF087434	0.16	25.275	22.789	0.18
NF-κB1	M57999	0.086	23.418	20.675	0.15
Stat6	L47650	0.064	24.546	21.152	0.095
CIITA	AF042158	0.048	38.072	21.096	7.8 × 10⁻⁶
Blimp-1	U08185	29.43	23.3	≥ 40	≥ 1.06 × 105
XBP-1	AW123880	17.15	17.492	21.738	18.97
PGK^1-153	M15668	1.13	21.397	21.588	1.14

PC indicates plasma cells; B, B cells; ND, not determined.

Calculated using normalized average difference values.

C_T indicates cycle number at which ΔRn crosses the threshold for that sample.

Calculated from the C_T difference between PC and B, assuming a 2-fold amplification per cycle.

PGK served as the positive control for both samples in the RT-PCR.

For TCF-1, refer to accession no. X61385 for PCR result.