Phenotype analysis of DCs matured in the presence of IVIg
| Treatment . | CD1a . | CD83 . | HLA-DR . | CD86 . | CD80 . | CD40 . | CD95 . |
|---|---|---|---|---|---|---|---|
| Ctl | 114 ± 22 | 21 ± 2 | 169 ± 35 | 62 ± 16 | 30 ± 12 | 295 ± 53 | 40 ± 12 |
| IVIg | 34 ± 15* | 12 ± 1* | 120 ± 52 | 36 ± 5* | 20 ± 9 | 95 ± 14* | 29 ± 10 |
| HSA | 124 ± 2 | 20 ± 3 | 154 ± 7 | 59 ± 6 | 25 ± 8 | 197 ± 34 | 37 ± 9 |
| Treatment . | CD1a . | CD83 . | HLA-DR . | CD86 . | CD80 . | CD40 . | CD95 . |
|---|---|---|---|---|---|---|---|
| Ctl | 114 ± 22 | 21 ± 2 | 169 ± 35 | 62 ± 16 | 30 ± 12 | 295 ± 53 | 40 ± 12 |
| IVIg | 34 ± 15* | 12 ± 1* | 120 ± 52 | 36 ± 5* | 20 ± 9 | 95 ± 14* | 29 ± 10 |
| HSA | 124 ± 2 | 20 ± 3 | 154 ± 7 | 59 ± 6 | 25 ± 8 | 197 ± 34 | 37 ± 9 |
Immature DCs of 5 day were cultured in the presence of IVIg (0.15 mM; IVIg) or the absence of IVIg (Ctl) for 48 hours. Equimolar concentration of HSA was used as control. Results are expressed as MFI, calculated by subtraction of the MFI obtained with the isotype-matched control value from that obtained with the relevant mAb. Results are from 4 independent experiments from different donors. Statistical significance as determined by unpaired Student t test is indicated.
P < .05.