Phenotype analysis of DCs differentiated in the presence of IVIg
| Treatment . | CD1a . | CD83 . | HLA-DR . | CD86 . | CD80 . | CD40 . |
|---|---|---|---|---|---|---|
| Ctl | 121 ± 27 | 21 ± 5 | 153 ± 26 | 55 ± 5 | 24 ± 4 | 282 ± 81 |
| IVIg | 31 ± 6* | 11 ± 2* | 59 ± 6* | 40 ± 5* | 21 ± 5 | 93 ± 15* |
| Treatment . | CD1a . | CD83 . | HLA-DR . | CD86 . | CD80 . | CD40 . |
|---|---|---|---|---|---|---|
| Ctl | 121 ± 27 | 21 ± 5 | 153 ± 26 | 55 ± 5 | 24 ± 4 | 282 ± 81 |
| IVIg | 31 ± 6* | 11 ± 2* | 59 ± 6* | 40 ± 5* | 21 ± 5 | 93 ± 15* |
Monocytes were cultured for 5 days with 1000 IU/mL rhGM-CSF and 500 IU/mL rhIL-4 in the presence of IVIg (0.15 mM; IVIg) or the absence of IVIg (Ctl). Results are expressed as MFI, calculated by subtraction of the MFI obtained with the isotype-matched control value from that obtained with the relevant mAb. Results are from 3 independent experiments from different donors. Statistical significance as determined by unpaired Student t test is indicated.
P <. 05.